A sensitive triple quadrupole mass spectrometer

描述（由申请人提供）：该资助的目的是购买一台三重四极杆质谱仪，以取代现有的10年之久的PE-Sciex API III仪器。该仪器将支持十七UAB教师和其他四个机构的六名教师的研究活动。所要求的仪器的优点是相当高的灵敏度（预计至少20倍，以允许在LC-MS中检测50 fmole），更现代化的计算操作系统和软件，以及由于从低温泵送系统切换到分子泵送系统而提高三倍的吞吐量。要求三重四极杆质谱仪使用多反应离子监测（MRM）提供最先进的定量LC-MS分析。它将在UAB、北方加州癌症中心、贝勒医学院、普渡大学和维克森林大学的一系列NIH资助的赠款中应用于多酚及其代谢物的分析。这些研究范围从使用细胞培养、啮齿动物和灵长类动物模型到临床试验。在UAB的一项研究中，将使用纳米电喷雾电离源测量组织间液。一个类似的来源将被用来帮助一组UAB研究人员谁希望应用蛋白质的定量分析方法。根据我们从Sciex API 3上的Protana接口构建的专用纳米电喷雾源的构建经验，应使用MRM分析定量测量低至1 fmole的浓度。预计到2003年秋季，将有一种用于nanoLC-MS分析的商用nanospray接口。

DESCRIPTION (provided by applicant): The objective of this grant is to purchase a triple quadrupole mass spectrometer to replace an existing 10-year old PE-Sciex API III instrument. This instrument will support the research activities of seventeen UAB faculty and six faculty at four other institutions. The advantages of the requested instrument are considerably higher sensitivity (expected at least 20-fold to allow detection of 50 fmole in LC-MS), a more modern computing operating system and software, and a three times higher throughput as a result of switching from a cryogenic pumping system to a turbomolecular one. The triple quadrupole mass spectrometer is requested to provide state-of-the-art quantitative LC-MS analysis using multiple reaction ion monitoring (MRM). It will be applied to analysis of polyphenols and their metabolites in a series of NIH-funded grants at UAB, the Northern California Cancer Center, Baylor College of Medicine, Purdue University, and Wake Forest University. These studies range from those using cell culture, rodent and primate models, to a clinical trial. In one study at UAB, measurement of interstitial fluids will be carried out using a nanoelectrospray ionization source. A similar source will be used to assist a group of UAB investigators who wish to apply methods for the quantitative analysis of proteins. Based on experience with the construction of a specialized nanoelectrospray source we constructed from the Protana interface on the Sciex API 3, as little as 1 fmole should be quantitatively measured using MRM analysis. It is anticipated that a commercial nanospray interface for use in nanoLC-MS analysis will be available by the fall of 2003.