Shrink Induced Manufacturing Platform for Low-Cost Diagnostics (SIMPL-CD)

描述（由申请人提供） 摘要：我的目标是通过开发极低成本、高特异性和超高灵敏度的塑料芯片，实现传染病的早期和快速检测。因此，唾液可以快速分析重要的蛋白质标记物与低功率，廉价的现成组件。早期、快速和定量检测将使病原体能够在患者所在地被识别，触发监测警报，给予适当的治疗，并遏制传染病。有了适当的工具，人们就可以确定不同的病原体菌株、耐药性模式和爆发源。发展中国家传染病的检测、诊断、治疗和监测对全民公共卫生至关重要。早期发现有助于及时和更有效的治疗，遏制疾病的传播，并减少无效反应的公共资源成本。这项提案的目标是开发和部署我的芯片，用于检测传染性和致命的轮状病毒疾病。虽然传统的光学检测是昂贵的，并且耗电量大的分析仪器不适合发展中国家的护理点（POC），我寻求提供一种低成本的便携式系统（含激光二极管和光电探测器，价格<100美元），带一次性耐用塑料芯片（具有用于表面增强传感的集成金属纳米结构），其制造成本低于$0.20美元一片就能为发展中国家提供真正的POC诊断。由于检测是基于抗体捕获，该设备可用于诊断几乎任何已知的传染病。这些模块化的基于微流体的组件也将允许多重检测，通过检查唾液中的一组蛋白质标记物来检测感染和合并感染。我在这些芯片中独特的集成纳米结构将信号增强了数千倍，从而实现了超高灵敏度的免疫测定。 公共卫生相关性：传染病的早期发现有助于及时和更有效的治疗，遏制疾病的传播，减少无效应对的公共资源成本，并提醒我们注意新出现的疾病。需要一种通用和现场加固的平台技术，该技术可广泛部署，但足够灵敏和强大，能够有效地在发病时检测特定感染和合并感染。

DESCRIPTION (Provided by the applicant) Abstract: My goal is to enable the early and rapid detection of infectious diseases by developing extremely low cost, high specificity and ultra-high sensitivity plastic chips. Saliva can thus be rapidly analyzed for important protein markers with low-powered, inexpensive off the shelf components. Early, rapid, and quantitative detection would enable pathogens to be identified at the patients' locale, triggering of surveillance alerts, administration of proper treatment, and containment of the infectious disease. With adequate tools, one could identify different pathogen strains, patterns of drug resistance, and sources of outbreaks. Detection, diagnosis, treatment, and surveillance of infectious diseases in the developing world is critical to universal public health. Early detection enables prompt and more effective treatments, contains the spread of diseases, and reduces the cost to public resources on ineffective responses. The goal of this proposal is to develop and deploy my chips for the detection of the infectious and deadly rotavirus disease. While traditional optical detection is expensive with power-hungry analytical instrumentations inappropriate for point of care (POC) in developing countries, I seek to provide a low-cost portable system (with laser diode and photo-detector for < $100) with disposable robust plastic chips (with integrated metallic nanostructures for surface enhanced sensing) that can be manufactured for less than $0.20 a piece would enable true POC diagnostics for developing countries. Because the detection is based on antibody capture, this device could be used to diagnose almost any known infectious disease. These modular microfluidic-based components would allow multiplex testing as well, enabling the detection of infections as well as co-infections by examining a panel of protein markers in saliva. My unique integrated nanostructures within these chips enhance the signal by several thousand folds, enabling ultra-high sensitivity immunoassays. Public Health Relevance: Early detection of infectious diseases enables prompt and more effective treatments, contains the spread of diseases, reduces the cost of public resources on ineffective responses, and alerts us of emerging diseases. A versatile and field-rugged platform technology that is widely deployable yet sensitive and robust enough to be efficacious in detecting specific infections and co-infections at their very onset is needed.