Photoreactive nanofibers for biomolecule immobilization

描述（由申请人提供）：该SBIR项目旨在展示和开发一种改进的纳米纤维组合物，特别适用于细胞培养和组织工程应用的表面改性和生物分子结合。一种新的三官能光活化交联剂将与现有的生物可降解聚合物一起形成表面含有光基团的纳米纤维。快速和容易的照明将使光化学键合到纤维表面，到适合于生物分子固定的聚合物或没有聚合物间隔物的生物分子本身。第二阶段的工作预计将开发用于组织工程的可生物降解支架，用于细胞组学和其他活细胞传感器系统的仿生细胞培养表面，为药物开发中的毒性和药物活性提供更可靠的评估。该阶段I提案的具体目标包括：1）优化用于制备光反应性聚合物纳米纤维的静电纺丝条件; 2）证明用生物分子结合间隔聚合物对光反应性纳米纤维的容易的表面改性;以及3）证明并优化在纳米纤维上固定具有上级功能负载密度的模型蛋白质链霉亲和素。

DESCRIPTION (provided by applicant): This SBIR project is designed to demonstrate and develop an improved composition for nanofibers especially suitable for surface modification and biomolecule binding for cell culture and tissue engineering applications. A new trifunctional photoactivatible crosslinker will be included with available biodegradable polymers to form nanofibers containing photogroups on the surface. Rapid and facile illumination will enable photochemical bonding to the fiber surfaces, to the polymers suitable for biomolecule immobilization or the biomolecules themselves without polymeric spacers. The Phase II work is expected to develop biodegradable scaffolds for tissue engineering, more biomimetic cell culture surfaces for cellomic and other living cell sensor systems providing more confident assessment of toxicity and pharmaceutical activities in drug development. Specific aims of this Phase I proposal include: 1) optimize the electrospinning conditions for preparing photoreactive polymer nanofibers; 2) demonstrate facile surface modification of the photoreactive nanofibers with biomolecule-binding spacer polymers; and 3) demonstrate and optimize immobilization on the nanofibers of a model protein, streptavidin, with superior functional loading density.