In-Vivo, Wireless, Fast-Scan Cyclic Voltammetry System

描述（由申请人提供）：本申请是Pinnacle Technology，Inc.团队申请1 R43 NS 050935 -01的重新提交。和堪萨斯大学的乔治·威尔逊博士将设计和建造一个无线、快速扫描循环伏安法（FSCV）系统，以监测自由活动大鼠体内的单胺神经递质。单胺（例如血清素、去甲肾上腺素和多巴胺）与许多严重的神经和精神障碍的病理学有关，包括帕金森病、阿尔茨海默病、双相情感障碍和精神分裂症。该系统将是一个有价值的工具，在体内研究的单胺类神经递质的测量。无线FSCV系统也将成为行为研究人员的主要资产。对自由活动的清醒动物的行为研究提供了行为和神经化学之间联系的关键知识。标准的FSCV规范将在这个支持蓝牙的无线系统中实现。也就是说，在-1.0V至+2.0V范围内的可调电压斜坡，用户定义的开始/结束点和扫描速率（< 1000 V/S），以及每秒至少10次的扫描重复率，以及每次扫描至少14位和至少1000点的同步模数转换。该系统将连续运行超过30小时的一套Vz AA锂电池（背部安装）。数据将传输到支持蓝牙的PC或笔记本电脑，以便在使用National Instruments LabView平台开发的自定义应用程序中进行分析。在阶段I期间将使用碳纤维电极（> 6 μ m），使用PBS和牛血清在体外测试系统。在第二阶段，将开发、测试和验证一个完整的交钥匙无线系统。将添加硬件和软件工具，以使FSCV扫描与外部刺激、行为研究视频或其他外部事件同步。

DESCRIPTION (provided by applicant): This application is a resubmission of Application 1 R43 NS050935-01 The team of Pinnacle Technology, Inc. and Dr. George Wilson of the University of Kansas will design and build a wireless, fast-scan cyclic voltammetry (FSCV) system to monitor monoamine neurotransmitters in freely moving rats. Monoamines (e.g. serotonin, norepinephrine, and dopamine) are implicated in the pathology of many severe neurological and psychiatric disorders, including Parkinson's disease, Alzheimer's disease, bipolar disorder, and schizophrenia. This system will be a valuable tool for the measurement of monoamine neurotransmitters in in vivo research. A wireless FSCV system will also be a major asset for behavioral researchers. Behavioral studies in freely moving, awake animals provide critical knowledge of the link between behavior and neurochemistry. Standard FSCV specifications will be implemented in this Bluetooth-enabled, wireless system. That is, an adjustable voltage ramp in the range of-1.0V to +2.0V, user defined start/end points and sweep rate (< 1000 V/S) , and sweep repetition rates of at least 10 per second, and synchronous analog to digital conversion of at least 14 bits and at least 1000 points per scan. The system will operate continuously for more than 30 hours on a single set of Vz AA lithium batteries (back mounted). Data will be transmitted to a Bluetooth enabled PC or laptop for analysis in a custom application developed using the National Instruments LabView platform. Carbon-fiber electrodes (> 6 urn) will be used during Phase I to test the system in vitro using PBS and bovine serum. During Phase II a complete turnkey, wireless system will be developed, tested and validated. Hardware and software tools will be added to synchronize the FSCV scans with external stimuli, video for behavioral studies or other external events.