ENDOCRINOLOGY CORE LABORATORY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在过去的一年里，ART核心为辅助生殖技术（ART）以及TNPRC的其他几个PI进行了RIA。所有RIA测定使用的试剂盒均购自Diagnostic Products Corporation。这些试剂盒包括标准品、I125标记的激素和与特异性抗体结合的试管。该测定是竞争性结合测定。基于结合至管的标记激素的量确定结果，并与标准品进行比较。在TNPRC开发的计算机程序用于建立最佳拟合曲线，并在测定后6至48小时内将结果提供给研究者，具体取决于测定的样品数量。试验数量包括测定雌二醇（1626次试验）、皮质醇（1338次试验）和孕酮（394次试验）的血清浓度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the past year the ART Core has conducted RIAs for the Assisted Reproductive Technology (ART) as well as several other PIs at the TNPRC. All RIA assays utilized kits were purchased from Diagnostic Products Corporation. These kits include standards, I125 labeled hormone, and tubes bound with specific antibody. The assay is a competitive binding assay. Results were determined based on the amount of labeled hormone bound to the tubes and compared to the standards. A computer program developed at the TNPRC was used to establish as ¿best fit¿ curve and results were provided to the investigators within 6 to 48 hours of the assay, depending on the number of samples assayed. The number of assays included determination of serum concentration for estradiol (1626 assays) cortisol (1338 assays) ) and progesterone (394 assays).