Tissue Procurement / Immunohistology

核心B(组织采购/免疫组织学)将作为已建立的组织的一部分运作， UPCI的研究病理服务(TARPS)资源。核心B将为所有四个项目提供支持 在本计划中，项目赠款。它将获取、加工、分发或存储来自患者的样本 癌症(口腔癌和其他HNC、黑色素瘤、前列腺癌或肾癌)或对照组， 例如，接受悬雍垂腭咽成形术或扁桃体切除术的非癌症患者作为HNC病例的对照。 样本，包括肿瘤活检组织以及肿瘤附近的正常组织，将从 患者或对照，由训练有素的人员根据既定的SOP或以下规定处理 严格的质量控制措施。标本将根据与美国卫生署建立的协议进行分类 参与调查，并将伴随着组织病理诊断和临床/人口统计学 数据。核心将向PPG调查人员提供冰冻组织、冰冻切片 常规石蜡包埋组织，塑料包埋切片。核心B将提供例程和 特殊病理服务包括免疫组织化学、放射自显影、原位杂交、电子 显微镜、定量图像分析和形态计量学以及数字成像和摄影。这个 CORE将处理新鲜或冷冻保存的标本，显微解剖组织，并准备RNA或DNA 需要的。匹兹堡大学的石蜡组织微阵列(TMA)设施是 油布，将提供给PPG调查人员。还将根据需要准备组织微阵列。堆芯 B将提供Core数据库中用于研究的每个样本的带注释的临床信息 并发挥“诚实经纪人”的作用，以满足HIPAA的要求。将在以下位置提供帮助 准备供标本使用和获取相关临床信息的IRB申请。

Core B (Tissue Procurement/lmmunohistology) will operate as a part of the established Tissue and Research Pathology Services (TARPS) Resource at the UPCI. Core B will provide support to all four projects in this Program Project Grant. It will procure, process, and distribute or store specimens from patients with cancer (oral carcinoma and other HNC, melanoma, prostate or renal cell carcinomas) or control individuals, e.g., non-cancer patients undergoing uvulopalatopharyngoplasty ortonsillectomy as controls for HNC cases. Specimens, including tumor biopsies as well as normal tissues adjacent to tumors will be collected from patients or controls, processed by trained personnel in accordance with the established SOP and under rigorous quality control measures. Specimens will be triaged according to the protocol established with the participating investigators and will be accompanied by histopathologic diagnoses and clinical/demographic data. The Core will make available to the PPG investigators snap frozen tissues, frozen sections, conventional paraffin embedded tissues, plastic embedding and sectioning. Core B will provide routine and special pathology services including immunohistochemistry, autoradiography, in-situ hybridization, electron microscopy, quantitative image analysis and morphometry as well as digital imaging and photography. The Core will handle fresh or cryopreserved specimens, microdissect tissues and prepare RNA or DNA as1 needed. The Paraffin Tissue Micro-Array (TMA) facility of the University of Pittsburgh, which is a part of TARPS, will be available to the PPG investigators. Tissue microarrays will also be prepared as needed. Core B will provide annotated clinical information on every specimen used in research from the Core database and function as an "honest broker" to meet HIPAA requirements. Assistance will be available in the preparation of IRB applications for the specimen use and access to associated clinical information.