FLOW CYTOMETRY SORTER FOR UNIV OF NC AT CHAPEL HILL: BIOCHEMISTRY

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请者提供)：我们正在申请资金购买达科细胞MoFlo分拣机。这台仪器是为了提高日益繁忙的北卡罗来纳大学教堂山流式细胞仪设备的分选能力，该设备是我们大型研究机构唯一提供研究流式细胞仪服务的供应商。该设施目前有5个分析流动细胞仪(2个FACScans、1个FACSCalibur、2个Cyans)和1个分选机。由于非常需要将于2005年3月下旬交付的第二台MoFlo分类器(本申请书中所述的仪器)，因此该大学已同意租用该仪器(没有购买选择权)，以便在获得购买该仪器的资金之前提供迫切需要的能力。该设施正在支持越来越多的NIH资助的研究人员，他们需要更多地获得这一关键研究技术。2004年，该设施提供了7351个小时的收费服务，其中1170个小时用于分拣。我们的服务支持16个不同部门和11个研究中心的140多个实验室(2004年使用了60个分拣)的研究，其中至少有5个是指定的NIH研究中心。所要求的仪器的能力将支持所有分选器用户的研究，但我们选择了21名调查员在这项申请中突出说明，作为正在进行的研究类型的代表。在接下来的一年里，预计所有用户总共需要这两台仪器80%以上的容量。我们选择MoFlo不仅是因为我们发现它是一种高度可靠的优秀仪器，还因为我们强烈地感觉到，拥有两台相同的分拣机在培训操作员、仪器之间的操作员互换、故障排除以及公司通信和服务方面为该设施提供了强大的优势。所要求的仪器将使用所有二极管/固态激光器提供3个激光激励、自动克隆、分拣室的气溶胶排空、数字电子、4路分选、11个荧光探测器和样品温度控制。我们认为这一仪器与公共卫生非常相关。及时、高质量、基础和可转化的生物医学研究是发展对疾病的理解和制定基于知识的合理干预战略的基础。流式细胞术是生物医学研究人员武器库中的一项强大工具，从而帮助他们实现生物医学研究目标。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are requesting funds to purchase a DakoCytomation MoFlo sorter. This instrument is needed to augment the sorting capacity of the increasingly busy University of North Carolina at Chapel Hill Flow Cytometry Facility, which is the only provider of research flow cytometry services at our large research institution. The Facility currently has 5 analytical flow cytometers (2 FACScans, 1 FACSCalibur, 2 CyAns) and one sorter. A second MoFlo sorter (the instrument described in this application), to be delivered in late March 2005, is so much needed that the University has agreed to lease (no purchase option) the instrument in order to provide the desperately needed capacity until funding can be secured to purchase the instrument. The Facility is supporting an ever-larger group of NIH-funded investigators needing increasing access to this key research technology. In CY2004 the Facility performed 7,351 hours of charged service of which 1,170 hours was for sorting. Our services support the research of over 140 laboratories (60 used sorting in CY2004) in 16 different departments and 11 research centers, at least 5 of which are designated NIH research centers. The capacity of the requested instrument will support the research of all sorter users but we have selected 21 investigators to highlight in this application as being representative of the types of research being performed. In total, all users are expected to need more than 80% of the capacity of both instruments in the upcoming year. We have chosen the MoFlo not only because we have found it to be a highly dependable, excellent instrument but also because we feel strongly that having 2 identical sorters provides strong advantages for the Facility in terms of training operators, interchange of operators between instruments, ease of troubleshooting and company communication and service. The instrument requested will provide 3 laser excitation using all diode/solid-state lasers, automated cloning, aerosol evacuation of sort chamber, digital electronics, 4-way sorting, 11 fluorescence detectors, and sample temperature control. We feel this instrument is very relevant to public health. Timely high quality basic and translational biomedical research is fundamental to the development of an understanding of disease and to the development of knowledge-based rational intervention strategies. Flow cytometry is a powerful tool in the arsenal of the biomedical researcher and, thus helps them realize their biomedical research goals.