FEI Tecnai 12 BioTWIN Transmission Electron Microscope

描述（由申请人提供）：约翰霍普金斯艺术与科学学院、工程学院、医学院和公共卫生学院的14名NIH资助的研究人员组成的多元化小组要求资金购买FEI Tecnai 12 BioTWIN透射电子显微镜（TEM）。所要求的TEM结合了针对高对比度优化的物镜透镜配置，非常适合我们的生物应用，包括传统的薄切片TEM;免疫金标记的冷冻切片;量子点;和生物聚合物可视化。重要的是，这种新的最先进的TEM将取代我们使用近30年的Philips EM 410 TEM，该TEM的服务（和部件）越来越成问题;未来的可靠性是一个主要问题，因为在前一年，当部件被定位以进行维修时，系统会导致停机时间延长。我们的研究人员，都通过NIH的资金，工作在一个主机的基本细胞生物学，生物化学和生物分子相关的问题，包括，但不限于：HSV组装和成熟; MHC I运输和组装，膜组织和动力学;内体/溶酶体动力学;膜运输;多聚基因载体的运输;线粒体和细胞凋亡;唾液酸展示和细胞粘附;和内吞作用的机制。新的Tecnai 12将确保我们不同的研究小组能够持续不间断地访问针对生物应用优化的TEM。Tecnai 12将位于生物系新装修的综合成像中心（IIC），这是一个霍姆伍德校园/霍普金斯大学范围内的显微镜资源，经常被多个学校和部门使用，包括60多个实验室和约200个用户。它将被纳入国际投资中心现有的完善的再充系统，以确保收回用于供应和维修/服务合同的资金。

DESCRIPTION (provided by applicant): A diverse group of 14 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health request funds to purchase a FEI Tecnai 12 BioTWIN transmission electron microscope (TEM). The requested TEM incorporates an objective lens configuration optimized for high contrast and is perfectly suited for our biological applications that include conventional thin section TEM; immunogold labeling of ultrathin cryosections; quantum dot; and biopolymer visualization. Importantly, this new state-of-the-art TEM will replace our well utilized near 30 year old Philips EM 410 TEM for which service (and parts) is becoming increasingly problematic; and future reliability a major concern with the system incurring extended downtimes during the previous year while parts are located to effect repair. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. The new Tecnai 12 will ensure that our disparate group of investigators have continued and uninterrupted access to a TEM optimized for biological applications. The Tecnai 12 will be conveniently located, administered, and maintained, in the Biology Department's newly renovated Integrated Imaging Center (IIC), a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. It will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance/service contracts.