CORE--Cell Analysis

描述(申请人提供)：细胞分析共享资源提供研究人员 获得流式细胞仪和细胞分选。细胞分析共享资源的目标是为所有感兴趣的研究人员提供全面的数据收集、数据分析、数据解释和流式细胞术方面的教育。这包括适当的样品准备以及对流式细胞术的理论和实践的教育。细胞分析共享资源提供了流动分析样本设置和处理的基本指导。这些包括：免疫荧光(1-4种颜色，1-7个参数)；标准CD标记的表面表达；定制抗体的表面标记的特异性表达；基因转导/表达；绿色荧光蛋白；受体表达； 这些研究包括：细胞凋亡；Annexin V结合；caspase活性；DNA分析；细胞周期分析；细胞凋亡；癌症治疗；动力学研究(时间依赖性分析)；细胞分选；细胞浓缩；细胞纯化；单细胞克隆；新方案开发；以及将新技术引入细胞分析共享资源现有服务库中的初步研究。细胞分析共享资源利用最新版本的分析软件提供对流式细胞仪数据的数据分析。

DESCRIPTION (provided by applicant): The Cell Analysis Shared Resource provides researcher access to flow cytometry and cell sorting. The goal of the Cell Analysis Shared Resource is to provide comprehensive data collection, data analysis, data interpretation and education in flow cytometry to all interested investigators. This includes proper sample preparation as well as education in theory and practice of flow cytometry. The Cell Analysis Shared Resource provides basic direction on the set up and processing of specimens for flow analysis. These include: immunofluorescence (1-4 colors, 1-7 parameters); surface expression of standard CD markers; specialized expression of surface markers with custom antibodies; gene transduction/expression; green fluorescent protein; receptor expression; apoptosis; Annexin V binding; caspase activity; DNA analysis; cell cycle assays; apoptosis; cancer therapy; kinetic studies (time dependent assay); cell sorting; cell enrichment; cell purification; single cell cloning; new protocol development; and preliminary studies to bring new techniques into the repertoire of available services of the Cell Analysis Shared Resource. The Cell Analysis Shared Resource provides data analysis of flow cytometric data utilizing the most current versions of analysis software.