MOLECULAR BIOLOGY /RECOMBINANT PROTEIN

描述(由申请人提供)：分子生物学核心已在 在过去的14年里，该公司为众多分子生物技术和重组蛋白的表达和纯化提供了最先进的方法学。分子生物学核心的运营资金来自UAB CFAR和所提供服务的按存储容量使用计费机制。随着核心的知名度和声誉的提高，综合癌症中心(CCC)成员在非CFAR项目中的使用已增加到核心活动的大约50%。这在一定程度上要归功于针对乳房和脑瘤孢子的CCC成员以及几个联合项目的成功。为了以负担得起的成本继续为CCC成员提供服务，我们建议建立一个联合CFAR/CCC核心，目标如下：具体目标1：培训人员(技术转让)，以协助重组蛋白的构建、聚合酶链式反应、突变和表达/纯化的各个阶段。具体目标2：以更低的成本为CCC成员提供以下合同服务：质粒DNA制备、亚克隆、重组蛋白表达、聚合酶链式反应和DNA突变。在过去14年的运作中，分子生物学核心平均每年援助20多名CCC成员。核心平均每年从CCC成员那里收取40,000美元的费用。最近，Core为从事乳房和脑瘤孢子项目的CCC成员提供专业知识和服务，以及UAB和SRI之间旨在开发癌症新自杀基因方法的联合项目。我们要求为核心人员提供支持，以保持对核心的多学科努力，并使所有CCC成员都能负担得起成本。所要求的大部分资金将是关键共享资源人员(大卫·麦克弗森)的工资，这与得到支持的CFAR核心人员(西尔维娅·麦克弗森)不同。

DESCRIPTION (provided by applicant): The Molecular Biology Core has been in operation for the past fourteen years and has provided state-of-the-art methodologies for numerous molecular biological techniques and recombinant protein expression and purification. Operating funds for the Molecular Biology Core have come from the UAB CFAR and through a chargeback mechanism for services rendered. As the visibility and reputation of the Core has increased, the use by Comprehensive Cancer Center (CCC) members on non-CFAR projects have increased to approximately 50% of the Core activities. This is due in part to the success of CCC members for Breast and Brain Tumor SPORES and several joint program projects. To keep servicing the CCC members at an affordable cost, we are proposing to establish a joint CFAR/CCC Core with the following goals: Specific Aim 1: To train personnel (technology transfer) to assist in the various phases of plasmid construction, PCR, mutagenesis and expression/purification of recombinant proteins. Specific Aim 2: To provide the following contract services to CCC members at reduced costs: Plasmid DNA preparation, subcloning, recombinant protein expression, PCR and DNA mutagenesis. During the last fourteen years of operation, the Molecular Biology Core has assisted on average over twenty CCC members per year. The Core has averaged $40,000/year in chargebacks from CCC members. Most recently, the Core has provided expertise and service to CCC members engaged in Breast and Brain Tumor SPORE and joint programs projects between UAB and SRI designed to develop new suicide gene methods for cancer. We are requesting support for Core personnel to maintain the multi-disciplinary efforts to the Core and to keep costs affordable to all CCC members. The majority of the requested money then will be salary for key shared resource personnel (David McPherson) that is distinct from the supported CFAR Core personnel (Sylvia McPherson).