NEIBank: Est Analysis And Bioinformatics For Ocular Geno

眼睛是一个由不同发育来源的高度分化的组织组成的复杂系统。许多对眼睛功能至关重要的基因是组织特异性的，其中许多已知的基因与遗传性眼病有关。大多数人类表达的基因都是通过表达序列标签(EST)才知道的。一个名为NEIBank的项目开始了，以提高科技报道的覆盖率，并开发一本分子眼科百科全书(http://neibank.nei.nih.gov).许多人类的眼睛(以及耳朵)组织和重要的动物模型的组织现在已经被分析。最近增加的包括人视神经、斑马鱼角膜、恒河猴和奶牛晶状体。本工作所产生的人类DNA资源已被用于构建包含13,000个非冗余人眼表达序列和6500个小鼠眼序列的基因芯片。眼睛研究信息学工具的一个强大补充是EyeBrowse，这是与CIT合作开发的专门用于眼睛表达基因的基因组浏览器。Http://eyebrowse.cit.nih.gov/genome/；是已知人类眼病基因的数据库，也是比较不同组织和物种表达模式的新工具。此外，NEIBank现在将来自EyeSAGE项目(Duke U)的数据与用于人眼来源的SAGE数据的定制基因组浏览器轨道相结合。另一项新增内容是人类眼病基因的全面数据库。

The eye is a complex system of highly differentiated tissues of various developmental origins. Many genes essential for eye function are tissue-specific and many of those known are associated with genetic eye diseases. The majority of human expressed genes are known only through expressed sequence tags (ESTs). A project called NEIBank was begun to improve the EST coverage and to develop a molecular encyclopedia for the eye (http://neibank.nei.nih.gov). Many human eye (and also ear) tissues and those of important animal models have now been analyzed. Recent additions include human optic nerve, zebrafish cornea and rhesus monkey and cow lens. The human DNA resource generated by this work has been used to create cDNA microarrays with 13,000 non-redundant human eye expressed sequences and 6500 sequences from mouse eye. A powerful addition to the informatics tools for eye research is EyeBrowse, a dedicated genome browser for eye-expressed genes, produced in collaboration with CIT. http://eyebrowse.cit.nih.gov/genome/; a database for known human eye disease genes and a new tool to compare expression patterns of different tissues and species. In addition, NEIBank now incorporates data from the EyeSAGE project (Duke U) with a custom genome browser track for human eye-derived SAGE data. Another addition is a comprehensive database of human eye disease genes.