Microsphere Coatings for Protein Arrays

描述(由申请人提供)：该SBIR项目旨在开发一种用于微阵列设备表面的通用且成本效益高的涂层技术，特别是用于蛋白质组和基因组分析。新型多功能光活化交联剂将用于微米和纳米粒子的共价薄膜固定化，开发出“虚拟势垒”条状、载玻片或平板阵列。亲水的微粒将形成固定化的生物分子分析部位，而疏水的纳米颗粒将形成“超疏水”纳米结构表面，分隔“虚拟井”分析部位。第二阶段的工作预计将开发一种密集阵列能力，利用自编码微粒在微阵列的每个位置或“地址”提供多分析物分析能力，确信显微镜载玻片阵列上至少有一百万个分析物能力。 这一第一阶段方案的具体目标包括：1)可重复性和低成本地合成一类新的寡官能性光反应交联剂及其在阵列载体表面通过共价薄膜产生的微粒子固定化的用途；2)通过将亲水性微粒子图案化地固定在由“超疏水”纳米织构表面分隔的分析位置来制备“虚拟井”阵列；以及3)展示由固定化微粒子形成的蛋白质组阵列相对于平面表面的优越特性。 这项拟议的工作有望产生新的微阵列涂层技术，该技术将在每个芯片的分析物容量(数量)、分析物分析灵敏度和样品应用简便性方面提供显著改进。第一阶段项目将生产一种新型的光反应成膜剂，用于涂覆各种诊断和可植入的医疗设备，包括为各种医疗、诊断和电子工业应用生成超疏水纳米结构表面。这一拟议项目对公众健康的好处将是更便宜和更准确的用于遗传和蛋白质组分析的微阵列。随着基因检测的增加和对药物相互作用的更具体的遗传倾向的了解，检测将需要更便宜和更可靠。此外，改进的微阵列将使研究蛋白质-蛋白质相互作用的研究人员受益，这最终可能极大地帮助疾病管理。

DESCRIPTION (provided by applicant): This SBIR project is designed to develop a versatile and cost-effective coating technology for surfaces used in microarray devices, especially for proteomic and genomic analyses. The "virtual well" strip, slide or plate array will be developed with new multifunctional photoactivatible crosslinking reagents for covalent thin film immobilization of micro- and nano-particles. The hydrophilic microparticles will form immobilized biomolecule assay sites and the hydrophobic nanoparticles will form "superhydrophobic" nanotextured surface separating the "virtual well" assay sites. The Phase II work is expected to develop a dense array capability utilizing self-encoded microparticles to provide multianalyte assay capability in each site or "address" of a microarray, with confidence of at least one million analyte capability on microscope slide array. Specific aims of this Phase I proposal include: 1) reproducible and cost-effective synthesis of a new class of oligofunctional photoreactive crosslinking reagents and their utility in immobilizing microparticles with covalent thin film generation on array support surfaces; 2) preparation of "virtual well" arrays via patterned immobilization of hydrophilic microparticles in assay sites separated by "superhydrophobic" nanotextured surfaces; and 3) demonstrate superior characteristics of proteomic arrays formed with immobilized microparticles vs flat surfaces. This proposed work is expected to generate new microarray coating technology which will provide significant improvements in analyte capacity (number) per chip, analyte assay sensitivity, and ease of sample application. The Phase I project will produce a new class of photoreactive film-forming reagents useful for coating a large variety of diagnostic and implantable medical devices, including the generation of superhydrophobic nanotextured surfaces for a variety of medical, diagnostic and electronic industry applications. The benefit of this proposed project to public health would be less expensive and more accurate microarrays for genetic and proteomic analysis. As genetic testing increases and more specific genetic predispositions to drug interactions are understood, the testing will need to be cheaper and more reliable. Additionally, the improved microarrays will benefit researchers studying protein-protein interactions which may ultimately greatly aid in disease management.