Microarray Technology for Genomic Assays

描述(由申请人提供)：对基因组规模的DNA序列信息的访问使为各种高通量分析设计越来越多的寡核苷酸(寡核苷酸)成为可能。从序列信息直接转化为合成寡核苷酸的能力一直是基因组学许多关键进展的基石。然而，尽管寡聚合成的成本已经大幅下降，但对于许多应用来说，它仍然过于昂贵。我们建议解决基因组学中的这一主要需求。这项第一阶段的提案旨在开发一种新的、廉价、高度灵活和高效的制造寡聚的方法。它适用于基因组学中的广泛应用。合成DNA被用于各种各样的分析来破译基因组。这项提议旨在开发制造和使用合成DNA的新的、高效的方法。这将扩大基因组学及其在医学进步中的应用范围。

DESCRIPTION (provided by applicant): Access to DNA sequence information on a genomic scale has enabled the design of ever-larger sets of oligonucleotides (oligos) for a wide variety of high-throughput assays. The ability to go directly from sequence information to synthetic oligos has been a cornerstone of many key advances in genomics. However, although the cost of oligo synthesis has decreased dramatically, it is still too expensive for many applications. We propose to address this major need in genomics. This Phase I proposal aims to develop a novel, inexpensive, highly flexible and efficient approach for manufacturing oligos. It is applicable to a wide range of applications in genomics. Synthetic DNA is used in a wide variety of assays to decode the genome. This proposal aims to develop new, highly efficient ways of making and using synthetic DNA. This will broaden the reach of genomics and its application to advances in medicine.