NMR STUDIES OF GLUCOSE METABOLISM IN CATS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。作为肥胖研究的一部分，猫在各种饮食条件下的葡萄糖代谢正在进行分析。通过跟踪同位素标记(碳-13和氢)并测量葡萄糖中特定原子位置的标记量，可以评估不同的代谢途径。分析包括从血浆中制备和提取葡萄糖衍生物(1，2-异亚丙基葡萄糖或单丙酮葡萄糖)，收集该化合物的碳-13和氢核磁共振谱，并测量相对峰强度。然后根据公布的算法对数据进行分析，以确定每个原子的来源。合作工作包括修改样品制备和核磁共振数据采集协议，以提高总体信号灵敏度，这是因为与已发表的对大鼠和人类的研究相比，CAT样品中可获得的血浆和同位素丰度较低。除了医学上的相关数据，该项目还将是分析型核磁共振的测试应用，并为毛细管流动探头等核磁共振硬件提供有用的基准数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Glucose metabolism in cats under a variety of dietary conditions is being analyzed as part of a study of obesity. By following isotope labels (carbon-13 and deuterium) and measuring amounts of label at specific atom sites in glucose, one can evaluate different metabolic pathways. The analysis involves preparing and extracting a glucose derivative (1,2-isopropylidene-glucofuranose or 'monoacetone glucose') from plasma, collecting carbon-13 and deuterium NMR spectra of this compound, and measuring relative peak intensities. The data are then analyzed according to published algorithms to identify the source of each atom. The collaborative work includes modifying sample preparation and NMR data acquisition protocols in order to increase overall signal sensitivity, due to the lower amounts of plasma and isotope enrichment available in the cat samples relative to published work on rats and humans. In addition to the medically relevant data, this project will be a test application of analytical NMR, and provide useful benchmark data for NMR hardware such as the capillary flow probe.