NMR STRUCTURAL ANALYSIS OF MAN10 AND MAN11/12

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。对三个样品的数据进行了分析，以确定是否存在-甘露糖基残留物。样品1：“man10”-这是描述为man9/10混合物的原始样品样品2：“man11”-这是描述为从P4馏分63-66中提取的man11/man12混合物的第二种制剂，估计含有50%的man11, 15%的man12。Sample3：“man10_digest”——这是第三种制剂，其中用α 1,2-甘露糖苷酶处理原始的man11/man12混合物以还原为man9或man10。还检查了含有核心锰8/锰9混合物的第四个样品。将样品从D2O中冻干，再溶解到200 uL或500 uL的D2O中。核磁共振数据在瓦里安Inova 600 MHz和800 MHz光谱仪上采集，温度为25℃。使用瓦里安库中的标准实验（梯度COSY、TOCSY、NOESY、梯度HSQC和HMBC）进行表征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Data from three samples were analyzed for the presence of beta-mannosyl residues. Sample 1: "man10" - this was the original sample described as a man9/10 mixture Sample 2: "man11" - this was a second preparation described as man11/man12 mixture from P4 fractions 63-66 with estimated 50% man11, 15% man12. Sample3: "man10_digest" - this was a third preparation wherein the original man11/man12 mixture was treated with alpha1,2- mannosidase to trim back to man9 or man10. A fourth sample containing the core man8/man9 mixture was also examined. Samples were lyophilized from D2O, and redissolved into 200 uL or 500 uL D2O. NMR data were collected on Varian Inova 600 MHz and 800 MHz spectrometers at 25¿C. Standard experiments from the Varian library (gradient COSY, TOCSY, NOESY, gradient HSQC and HMBC) were used for characterization.