Fold recognition by synchrotron radiation circular dichroism spectroscopy: a new tool for structural proteomics and structural molecular biology

蛋白质的三维结构决定了它们的功能特性。目前，圆二色性光谱方法可用于在二级（局部）结构水平上检测溶液中蛋白质的结构。这项建议是开发一种新的方法，以扩大信息内容使用较低的波长数据。这种方法将为英国和国外正在开发的结构蛋白质组学新项目所产生的信息提供补充信息，特别是因为它将提供来自非常少量蛋白质（包括那些不结晶的蛋白质）的结构和折叠基序水平的结构信息。此外，由于英国已经投资了一个新的第三代同步加速器光源，钻石，它将有一个圆二色性站作为其早期的光束线之一，这一建议将提供一个新的应用和手段，从收集的数据中提取额外的信息光束线。

The three dimensional structures of proteins define their functional properties. At the present time, circular dichroism spectroscopic methods can be used to examine the structures of proteins in solution at the level of secondary (local) structure. This proposal is to develop a new methodology to extend the information content using lower wavelength data. Such a method will provide complementary informtion to that produced by new programmes in structural proteomics being developed in the UK and abroad, especially as it will provide structural information at the level of architecture and fold motifs to be derived from very small amounts of proteins, including those that do not crystallise. In addition, as the UK has invested in a new third-generation synchrotron light source, Diamond, which will have a circular dichroism station as one of its early beamlines, this proposal will provide a new application and means of extracting extra information from the data collected on that beamline.