MALDI & ESI & LC ESI QQTOF MS TRAINING

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们的实验室广泛使用应用生物系统API QSTAR（tm）脉冲星i QqTOF质谱仪来表征从合作研究项目中获得的复杂生物样品。在本报告年度，向几名工作人员提供了关于设计和使用QSTAR的培训，以便通过ESI-MS/MS、MALDI-MS/MS和LC-ESI-MS/MS分析样品。他们接受了批处理参数文件、处理脚本和针对典型蛋白质消化优化的自动化IDA MS/MS功能的使用培训。提供了色谱指导，包括色谱柱填充方法的理论和应用，色谱分离参数和ESI界面。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory makes extensive use of the Applied Biosystems API QSTAR(tm) Pulsar i QqTOF mass spectrometer for the characterization of complex biological samples obtained from collaborative research projects. During the current reporting year, several staff members have been provided training on the design and use of the QSTAR for the analysis of samples via ESI-MS/MS, MALDI-MS/MS and LC-ESI-MS/MS. They have been trained in the use of batch parameter files, processing scripts and automated IDA MS/MS functions that have been optimized for typical protein digests. LC instruction has been provided and includes theory and application of column packing methodology, LC separation parameters and ESI interfaces.