Sequencing the genome of the powdery mildew fungus Blumeria graminis

这项建议旨在获得白粉菌Blumeria graminis f sp hordei的注释基因组序列。该真菌是一种专性生物营养病原菌，在农学上是大麦最重要的病害之一，是研究其他作物白粉病的模式生物。我们将对基因组进行7倍的测序。该序列将被自动组装。还将对从吸器中分离的cDNA的EST文库进行测序和注释；这将补充现有的收藏，并完成对病原体生命周期所有重要阶段的覆盖。EST在训练驱动注释的基因发现过程中起着重要作用。基因组序列的注释将使用一系列已经用于其他基因组项目(Botrytis和番茄)的管道进行。半自动注释的结果随后将通过人工管理进行验证，并由测序联盟中的专业研究人员进行审查。结果将放在公众可访问的网站上，并将与世界各地的用户社区召开一次会议，培训潜在用户，介绍排序和第一次注释的主要结果，并讨论结果。

This proposal aims to obtain an annotated genome sequence of the powdery mildew fungus Blumeria graminis f sp hordei. This fungus is an obligate biotrophic pathogen that causes one the agronomically most important diseases of barley and is a 'model' organism for the study of powdery mildew diseases in other crops. We will sequence the genome with a 7-fold coverage. The sequence will be assembled automatically. An EST library of cDNAs isolated from haustoria will also be sequenced and annotated; this will complement the existing collections and complete the coverage of all the important stages in the pathogen's life cycle. The ESTs are instrumental in training the gene finding processes that drive the annotation. The annotation of the genomic sequence will be carried out using a series of pipelines already used for other genome projects (Botrytis and tomato). The results from the semi-automated annotation will then be verified by manual curation and reviewed by specialist researchers in the sequencing consortium. The results will be placed on publicly accessible web-sites and a meeting will be convened with the world wide user community to bring train potential users, present major outcomes of the sequencing and the first annotations and discuss the results.