EFFECT OF SYSTEMIC HYPOXIA ON MSNA & ITS RELATIONSHIP TO INTERSTITIAL ADENINE NU

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。在全身缺氧期间，交感神经活动增加，伴随着骨骼肌间质腺苷和去甲肾上腺素的增加。尚不清楚间质腺苷的增加是否来自血管内皮、骨骼肌纤维或ATP的细胞外转化。在这项研究中，我们提出，通过同时测量去甲肾上腺素和腺嘌呤核苷酸（ATP，ADP和AMP）从肌内微透析探针在三个明显不同的sypatho兴奋性干预，我们将获得必要的区分独特的血管反应缺氧。将使用的三种不同的15分钟刺激包括：1）全身缺氧; 2）硝普钠静脉输注; 3）下体负压。将有16名志愿者参与研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During systemic hypoxia, sympathetic nerve activity is increased accompanied by increases in skeletal muscle interstitial adenosine and norepinephrine. It is not known whether the increases in interstitial adenosine come from vascular endothelium, from skeletal muscle fiber or from extra cellular conversion of ATP. In this study, we propose that through simultaneous measurement of norepinephrine and adenine nucleotides (ATP, ADP, and AMP) from intramuscular microdialysis probes during three distinctly different sypatho excitatory interventions we will obtain the necessary to differentiate unique vascular responses to hypoxia. The three different 15 minute stimuli that will be used include: 1) systemic hypoxia; 2) intravenous infusion of sodium nitroprusside; and 3) lower body negative pressure. 16 volunteers will be studied.