MRI CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在OMRF和OUHSC，我们利用高分辨率磁共振技术(包括显微成像和光谱)进行生物医学研究。啮齿动物实验动物模型的使用极大地提高了我们分析和理解各种疾病的分子基础的能力，但很少有方法能够以任何有意义的分辨率在活体和非侵入性地显示啮齿动物的各种组织、器官和血管系统。以下仪器是Bruker Biosin Biospec 70/30 usr卧式磁性小型动物成像光谱仪，能够获得啮齿动物实验动物模型的活体显微图像(分辨率~100？m或更高的显微MRI)，此外还提供关于分子靶标的在体功能信息(增强MRI)以及代谢产物的结构信息，如脂质/磷脂、生物含能化合物、肌酸、胆碱和乳酸(使用磁共振光谱(MRS))。磁共振成像(MRI)及其在生物医学研究中的相关技术，如磁共振血管成像(MRA)、磁共振波谱(MRS)在过去几年中的发展，在高场磁体、高强度磁场梯度、成像探针设计和组织特异性造影剂方面取得了重大进展，使得在小鼠和大鼠身上进行选择性的形态、功能和代谢研究成为可能。磁共振技术的另一个最新进展是磁共振显微镜的发展。可以获得优于100微米的面内分辨率的图像，并且可以收集具有以前只有窄口径液体核磁共振光谱仪才能获得的灵敏度和光谱分辨率的活体光谱。目前，我们的设施中使用形态MRI和/或显微MRI(~100？m分辨率)来定位和确定大鼠模型(Towner)中的脑癌病变以及大鼠和/或黑色素瘤(Chen)模型中的乳腺癌和/或黑色素瘤病变，并使用相敏和化学位移成像(CSI)方法监测激光诱导肿瘤消融中的温度变化(Chen)。磁共振波谱(MRS)，使用图像引导的MRS，也被用来监测大鼠脑瘤发病机制的代谢特征(脂质和脑代谢物改变；唐纳)，以及评估治疗药物(汤纳)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. At OMRF and OUHSC we utilize high-resolution MR techniques (including microscopic imaging and spectroscopy) for biomedical research. The use of rodent experimental animal models has dramatically advanced our ability to analyze and understand the molecular basis of various diseases, however few methods exist to be able to visualize the various tissues, organs and vascular systems of rodents in vivo and non-invasively at any meaningful resolution. The following instrument, a Bruker Biospin Biospec 70/30 USR horizontal magnetic small animal imaging spectrometer, is capable of obtaining in vivo microimages (at ~100 ¿m resolution or better for microscopic MRI) in rodent experimental animal models, and in addition provide in vivo functional information on molecular targets (contrast-enhanced MRI) as well as structural information on metabolites, such as lipids/phospholipids, bioenergetic compounds, creatine, choline, and lactate (using magnetic resonance spectroscopy (MRS)). Magnetic resonance imaging (MRI), and its related techniques in biomedical research, such as MR angiography (MRA), MR spectroscopy (MRS), have developed over the past few years with significant advances in high-field magnets, high-strength magnetic field gradients, imaging probe designs and tissue-specific contrast agents, which have allowed selective morphological, functional and metabolic investigations in mice and rats to be possible. Another recent advancement in MR technology has been the development of MR microscopy. Images can be obtained with in-plane resolutions better than 100 micron, and in-vivo spectra can be collected with sensitivity and spectral resolution previously obtained only by narrow-bore liquid NMR spectrometers. Morphological MRI and/or microscopic MRI (~100 ¿m resolution) is currently used in our facility to localize and determine the extent of brain cancer lesions in a rat model (Towner) and breast and/or melanoma cancer lesions in rats and mice (Chen), and to monitor temperature changes in laser-induced tumor ablation with the use of phase-sensitive and chemicla-shift imaging (CSI) methods(Chen). Magnetic resonance spectroscopy (MRS), using image-guided MRS, is also used to monitor metabolic profiles for rat brain tumor pathogenesis (lipid and brain metabolite alterations; Towner), as well as assess therapeutic agents (Towner).