IMPROVED MASS MEASUREMENT IN FTMS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。准确的质量测量能力是傅里叶变换质谱仪(FTMS)的一个重要属性。与质谱学中的其他仪器方法不同，FTMS仍然提供了提高质量测量精度(MMA)的重要机会，使其成为一个研究领域。尽管FTMS可以测量到九位数的离子频率，但频率到m/z的转换是困难的，因为频率取决于离子陷阱中的电场和空间电荷。我们正在研究填补电场(电气补偿)和修正空间电荷效应的方法。我们还对质量校准与离子运动和空间电荷效应的基本原理之间的关系感兴趣，我们希望改进基本的校准程序，特别是那些校正空间电荷效应的程序。基质辅助激光解吸电离(MALDI)和电喷雾电离(ESI)的出现为FTMS用于分析包括蛋白质、寡核苷酸(ODN)、寡糖和合成聚合物在内的高性能生物聚合物打开了大门。我们将在这些领域追求准确的质量测量，并寻求一些切实可行的方法来改进MMA。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The capability for accurate mass measurements is an important attribute of Fourier transform mass spectrometry (FTMS). Unlike other instrumental methods in mass spectrometry, FTMS still offers significant opportunities to improve mass measurement accuracy (MMA), making it an area of research. Although FTMS can measure ion frequencies to nine significant figures, the conversion of frequency to m/z is difficult because frequency depends on the electric field and the space charge in the ion trap. We are working on methods to shim the electric field (electrical compensation) and to correct for space charge effects. We are also interested in the relation of mass calibration and the fundamentals of ion motion and space-charge effects, and we wish to to improve the basic calibration procedure particularly those that correct for effects of space charge. The advent of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) have opened the door for FTMS to be used for analyzing at high performance biopolymers, including proteins, oligodeoxynucleotides (ODNs), oligosaccharides, and synthetic polymers. We will pursue accurate mass measurement in these areas and some practical ways to improve MMA.