900 MHz NMR Spectrometer for Structural Biology

描述：纽约结构生物学中心是一家501(c)(3)公司，由代表九个研究机构的董事会管理，建议购买一台900 MHz核磁共振光谱仪用于结构生物学研究，并寻求用于光谱仪成本的液体。主要研究人员和其他研究人员将分享使用这一资源，并鼓励其他用户。要构建的研究包括蛋白质和核酸的结构测定、动力学研究、蛋白质折叠、同位素标记方法的开发、高分子量应用和膜蛋白应用。这些研究是项目的一部分，旨在理解结构生物学的基本科学问题，以及涉及许多发育疾病、癌症、肌肉骨骼疾病、神经系统疾病和传染病的生理和病理过程。研究人员包括使用核磁共振的领先结构生物学家，以及该领域领先的实验家和方法开发人员。这种丰富的知识环境将有利于科学生产力、创造力和使用所提议的仪器的协作。900兆赫的光谱仪将被安置在一个10000平方米的空间里。如果。扩建部分目前正在建设中，与NYSBC现有的实验室相邻。研究人员将可以使用800兆赫及以下的现场仪器，因此900兆赫系统可以选择性地用于需要超高场的实验人员。超高场的特殊要求包括使用横向优化弛豫光谱（TROSY）及其衍生物、测量残余偶极耦合的磁取向、场相关CSA、动力学和其他弛豫相关现象在大分子量系统中的应用。

DESCRIPTION: The New York Structural Biology Center, a 501(c) (3) corporation governed by a Board representing nine research institutions, proposes to purchase a 900 MHz NMR spectrometer for research in structural biology, and seeks fluids for art of the Cost of the spectrometer. The principal investigators and other investigators will share in the use of this resource, and will encourage other users. Research to be constructed includes structure determination of proteins and nucleic acids, dynamics studies, protein folding, and development of methods of isotopic labeling, application to higher molecular weights, and application to membrane proteins. These studies are part of programs aimed at understanding both basic scientific problems in structural biology and at physiological and pathological processes involved in many diseases of development, cancer, muscle skeletal disorders, neurological diseases, and infectious diseases. The investigators include leading structural biologists using NMR, and leading experimentalists and methods developers in the area. This rich intellectual environment will be fruitful for scientific productivity, creativity, and collaboration using the proposed instrument. The 900 MHz spectrometer will be housed in a 10,000 sq. if. extension now in construction, contiguous with the NYSBC's existing laboratory. The investigators will have access to' 800 MHz and lower field instruments, so that the 900 MHz system Can be used selectively for those experimentalists requiring ultra high field. Specific requirement S for ultra high field include applications to large molecular weight systems using transverse optimize relaxation spectroscopy (TROSY) and its ''derivatives, magnetic orientation for measurement of residual dipolar couplings, and field-dependent CSA, dynamics, and other relaxation-related phenomena.