A Novel Combination of Thermoresponsive and Nanofibrillar Surface for Cell Cultur

描述（由申请人提供）：这项SBIR I期研究旨在开发和提供细胞培养市场，显著改善细胞生长，分化控制和收获的表面。将展示两种最近证明的改进的组合（热响应聚合物，可使细胞/组织从支持表面轻松温和地释放，纳米纤维纹理可改善细胞粘附性和天然特性表达），并与当前的商业细胞培养表面进行比较。用于这种纹理表面的纳米纤维将被静电纺丝或光化学涂覆热敏聚合物。薄涂层或用热敏聚合物纺丝纳米纤维将允许干细胞和其他哺乳动物细胞在更像天然组织的环境中培养，并允许细胞和细胞片的快速分离，而不会受到胰蛋白酶或机械刮擦释放处理的损害。因此，这种新的细胞培养表面技术提供了一种温度响应的仿生表面，在组织工程、细胞治疗、细胞组学（用于毒性测定的细胞阵列）和细胞-基质相互作用研究中具有潜在的应用前景。该项目将开发用于在更明确、无疾病和原生组织样生长环境中培养和收获哺乳动物细胞的表面。这种具有温度响应释放聚合物的纳米纹理表面将使细胞作为个体或薄组织的生长和控制分化以及它们从生长表面释放而不会受到消化酶或机械刮伤的损害。细胞培养技术的这一重大改进将为基础和临床研究增加巨大的价值，从而导致细胞治疗，在这些治疗中需要现成的精选和健康细胞供应。

DESCRIPTION (provided by applicant): This SBIR Phase I study is designed to develop and provide to the cell culture market, significantly improved surfaces for cell growth, differentiation control, and harvesting. The combination of two recently demonstrated improvements (thermally-responsive polymer for facile and gentle cell/tissue release from the support surface and nanofibrillar texture for improved cell adhesion and native property expression) will be demonstrated and compared with current commercial cell culture surfaces. The nanofibers for this textured surface will be electrospun or photochemically coated with thermoresponsive polymer. Thin coating or spinning the nanofibers with thermoresponsive polymer will allow the culture of stem and other mammalian cells in a more native tissue-like environment and allow rapid detachment of cells and cell sheets without the damage from trypsin or mechanical scraping release treatment. Thus, this proposed new cell culture surface technology offers a temperature-responsive biomimetic surface with potential applications in tissue engineering, cell therapy, cellomic (cell arrays for toxicity assays) and cell-matrix interaction studies.Project Narrative This project will develop surfaces designed for the culture and harvest of mammalian cells in a more defined, disease-free and native tissue-like growth environment. This nanotextured surface with temperature- responsive release polymer will enable the growth and controlled differentiation of cells as individuals or thin tissues and their release from the growth surface without damage from digestive enzymes or mechanical scraping. This significant improvement in cell culture technique will add great value to basic and clinical research leading to cell therapy where a ready supply of selected and healthy cells are needed.