DOPAMINERGIC MODULATION OF NETWORK ACTIVITY IN BASOLATERAL AMYGDALA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 与纽约哥伦比亚大学的弗吉尼亚·皮克尔博士合作，研究了多巴胺(DA)受体激活对杏仁基底外侧核(BLA)谷氨酸传递的影响。我们结合电子显微镜和电生理技术证明，在BLA投射神经元的突触后膜上，NMDA受体和多巴胺D1受体之间存在直接的蛋白质-蛋白质相互作用，其功能是减弱NMDA受体介导的突触后电流。此外，我们还证明了进入BLA的谷氨酸能传入的亚群也同时表达NMDA和D1受体，这表明突触前终末也可能发生蛋白质-蛋白质相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In collaboration with Dr Virginia Pickel of Columbia University, New York, the effects of dopamine (DA) receptor activation on glutamate transmission in the basolateral amygdala (BLA) were examined. We combined electron-microscopic and electrophysiological techniques to demonstrate that in the postsynaptic membrane of BLA projection neurons a direct protein-protein interaction occurs between NMDA receptors and dopamine D1 receptors, which functions to attenuate the NMDA receptor-mediated postsynaptic currents. Moreover, we demonstrated that a subpopulation of glutamatergic afferents into the BLA also co-express both NMDA and D1 receptors, suggesting that a protein-protein interaction may also occur in presynaptic terminals.