DIG FREQ DOMAIN HETERODYNING FLUOR LIFETIME ACQUISITION MODULE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 激光扫描显微镜系统数字频域外差荧光寿命采集模块 本课题基于现场可编程门阵列技术，设计并实现了一种用于激光扫描共焦显微镜的荧光寿命采集模块。测量荧光寿命的基本挑战是在纳秒时间尺度上准确确定激发和发射之间的时间。该芯片允许实现100 MHz数量级的数字频域外差，以测量荧光寿命衰减，与模拟频域技术相比，具有更好的占空比和易用性，并且成本比时域技术低得多。介绍了数字频域外差法的工作原理。该操作在特定的现场可编程门阵列芯片中实现，并用于商用激光扫描显微镜。初步结果包括对已知寿命样本的测量，以说明该器件的灵敏度和噪声特性。未来的工作将延伸到生物学应用，以演示用荧光寿命成像显微镜(FLIM)确定细胞活动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. FULL TITLE: Digital Frequency Domain Heterodyning Fluorescence Lifetime Acquisition Module for Laser Scanning Microscopy System This project is to design and implement a fluorescence lifetime acquisition module for laser scanning confocal microscopes based on Field Programmable Gate Array (FPGA) technology. The basic challenge of measuring fluorescence lifetime is to accurately determine the time between the excitation and the emission on the nanosecond timescale. The FPGA chip allows the implementation of digital frequency-domain heterodyning on the order of 100MHz to measure fluorescence lifetime decays with an improved duty-cycle and ease of use over analog frequency-domain techniques, and significantly lower cost than time-domain techniques. The principle of the digital frequency domain heterodyning method is described. The operation was implemented in a particular FPGA chip and used in a commercial laser scanning microscope. Preliminary results include the measurement of known lifetime samples to illustrate the sensitivity and noise characteristics of this device. Future work will extend to the biological applications to demonstrate the determination of cellular activities with fluorescence lifetime imaging microscopy (FLIM).