CHARACTERIZATION OF NOVEL BACTERIAL AGENTS ISOLATED FROM LABORATORY ANIMALS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 摘要：在实验啮齿动物体内发现了几种新的细菌种类。值得注意的是，我们已经确定了螺杆菌的新物种和几种尚未分类的细菌；后者被称为纤毛相关呼吸道(CAR)杆菌。为了进一步确定这些细菌和其他新细菌的特征，我们常规地对16S rRNA基因进行测序，并使用这些序列进行分类。我们还经常使用聚合酶链式反应片段的序列分析来确认反应的特异性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Abstract: Several novel bacterial species have been identified in laboratory rodents. Notably, we have identified novel species of Helicobacter and several species of bacteria that have not been classified; the latter are referred to as cilia-associated respiratory (CAR) bacilli. To further characterize these and other novel bacteria, we routinely sequence the 16S rRNA gene and use these sequences for classification. We also frequently employ sequence analysis of PCR fragments to confirm specificity of reactions.