CHARACTERIZATION OF YPKA PATHOGENICITY IN HOST CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我计划与本·吉普曼一起研究YpkA的致病性，YpkA是耶尔森氏菌的一种效应蛋白。目前，已知YpkA负责肌动蛋白细胞骨架一旦进入宿主细胞后的溶解。然而，到目前为止，这种情况发生的机制尚不清楚。我们计划将YpkA载体导入或注射到稳定表达GFP-肌动蛋白或mCherry-肌动蛋白的宿主细胞中。通过使用共聚焦显微镜，我们可以更清楚地解析致病蛋白的时空分布以及由此导致的肌动蛋白破坏。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I plan to work with Ben Giepmans to characterize the pathogenicity of YpkA, a Yersinia effector protein. Currently, it is know that YpkA is responsible for the dissolution of the actin cytoskeleton once it has been introduced into host cells. The mechanism by which this occurs, however, is thus far unknown. We plan to transfect or microinject YpkA plasmids into host cells that stably express GFP-actin or potentially mCherry-actin. Through the use of confocal microscopy, we can then resolve more clearly the spatio-temporal distribution of the pathogenic protein as well as the resulting actin disruption.