Arrays for Protease Activity Profiling

描述(由申请人提供)：需要新的技术来实现对蛋白酶活性的高分辨率、多路分析。这一领域的进展有可能使对细胞功能和调节的基本新见解的发现成为可能。我们建议开发一种新的技术，利用蛋白酶底物多肽芯片来灵敏和特异地分析蛋白酶活性。我们将开发针对高通量蛋白酶活性分析进行优化的阵列制备、多肽标记和检测方法。 公共卫生相关性：我们正在开发的蛋白酶活性分析系统将有助于破译细胞对蛋白质降解的调节以及与表型的关系。它将有助于识别导致疾病的细胞调节障碍，从而可能导致疾病诊断和治疗的医学进步。

DESCRIPTION (provided by applicant): There is a need for new technologies to enable high-resolution, multiplexed analysis of protease activity. Advances in this area have the potential to enable the discovery of fundamental new insights into cellular function and regulation. We propose to develop a novel technology for sensitive and specific analysis of protease activity using protease substrate peptide microarrays. We will develop methods for array preparation, peptide labeling and detection that are optimized for high-throughput protease activity analysis. PUBLIC HEALTH RELEVANCE: The system we are developing for protease activity analysis will help to enable the deciphering of cellular regulation of protein degradation and the relationship to phenotype. It will help to identify disorders in cellular regulation that lead to disease, and therefore may result in medical advances in the diagnosis and treatment of disease.