Portable Oxygen Gas Perfusion System for Pancreas Preservation

描述(由申请人提供)：拟议工作的目标是开发一种实质上改进的保存人类胰腺的技术，以提高从其获取的分离的人类胰岛的质量，用于人类胰岛移植治疗1型糖尿病。目前，人胰腺的运输采用两层法(TLM)储存(8℃)，这种方法使用含氧全氟碳溶液作为外部氧气储存库。虽然TLM被广泛报道为改善胰岛质量，但在获得可行的胰岛数量方面仍存在不足。这部分归因于在胰腺储存过程中可存活的胰岛丢失。初步计算和实验表明，胰腺的体外氧合只有利于器官外缘的组织，使胰腺的大部分体积没有氧合(85%-95%)。这项拟议的工作将使用一种新的方法将氧气输送到器官内部。这项研究和开发的另一个创新部分是开发一种系统，利用便携式电化学氧气浓缩器/发生器产生和调节向器官输送的氧气。建议的电化学氧气浓缩器使用电力从空气中浓缩氧气，并将其释放为纯净、潮湿的氧气，可以与环境空气混合，为建议的保存技术提供最有效的pO2。向器官内部输送气态氧气比器官全液体灌流要简单得多。此外，拟议的系统将被设计为便携式的，因为胰腺在分离胰岛之前的主要“储存”时间是在将其运送到专门从胰腺采集胰岛的设施期间。氧气将在所需的流速和压力下持续产生，不需要运输加压气瓶。这种拟议的方法有可能实现从胰腺收获的胰岛质量的突破，而不是渐进的改善。通过胰岛移植有效治疗重症I型糖尿病是尖端医疗研究的高度优先事项，拟议的工作将有助于该领域的进步。商业潜力保存系统设备可以是一种特殊的商业产品，供应给主要的器官采集和移植中心。或者，使用拟议的技术运输和保存胰腺可以是一项商业服务。该技术和设备可应用于其他器官的保存。胰腺；胰腺；1型糖尿病；器官转运；胰岛移植；电化学氧浓度。

DESCRIPTION (provided by applicant): The goal of the proposed work is to develop a substantially improved technique of preserving the human pancreas in order to improve the quality of the isolated human islets that are harvested from it for use in human islet transplantation for Type 1 diabetes. Currently, human pancreata are transported with the two layer method (TLM) of storage (8¿C), which uses an oxygenated perfluorocarbon solution as an external oxygen reservoir. While the TLM is widely reported to improve islet quality, there are still insufficiencies in the number of viable islets acquired. This is partially attributable to viable islet loss during pancreas storage. Preliminary calculations and experiments indicate that external oxygenation of the pancreas only benefits tissue in the outer rim of the organ leaving the majority of the volume of the pancreas without oxygenation (85-95%). The proposed work will use a novel method of oxygen delivery to the interior of the organ. Another innovative component of the research and development is to develop a system to generate and regulate oxygen delivery to the organ using a portable electrochemical oxygen concentrator/generator. The proposed electrochemical oxygen concentrator uses electrical power to concentrate oxygen from air and release it as pure, humidified oxygen that can be blended with ambient air to give the most efficacious pO2 for the proposed preservation technique. Delivery of gaseous oxygen to the interior of the organ is less complex than full liquid perfusion of the organ. Also the proposed system will be designed to be portable since the main "storage" time of the pancreas before islet isolation is during its transportation to a facility that specializes in harvesting islets from pancreata. Oxygen will be continuously generated at the desired flow rate and pressure without the need for transporting pressurized gas cylinders. This proposed method has the potential to effect a breakthrough, rather than an incremental improvement, in the quality of islets harvested from the pancreas. The effective treatment of serious cases of Type I Diabetes through pancreatic islet transplant is a high priority in cutting-edge healthcare research, and the proposed work will contribute to the advancement of the field. COMMERCIAL POTENTIAL The preservation system equipment could be a specialty commercial product, supplied to the major organ harvest and transplantation centers. Alternatively, the transportation and preservation of pancreata, using the proposed technique, could be a commercial service. The techniques and equipment could be applicable to the preservation of other organs. KEYWORDS pancreas, pancreata, Type I Diabetes, organ transport, islet transplantation, electrochemical oxygen concentration.