DEVELOPMENT OF REVERSIBLE INACTIVATION TECHNIQUE STUDY HIGHER COGNITIVE FUNCTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在这项研究中，我们使用了最先进的可逆失活技术，更详细地探讨了猴子记忆功能中内侧颞叶不同组成部分之间的相互作用。这些方法允许内侧颞叶内的单个结构在短时间内选择性地“关闭”和“打开”，同时测试某些特定的记忆功能。 这些可逆的失活过程产生“虚拟损伤”，因为潜在的神经回路在结构上没有被破坏，并且特定的位点只是暂时失活。 在报告期内，我们对一只成年雄性猴子进行了行为训练，以测量识别记忆能力。 在一半的测试阶段中，用蝇蕈醇（GABA激动剂）灭活靶结构，另一半注射载体。 我们的初步数据表明蝇蕈醇在25分钟至1小时之间达到最大的行为效应，之后效应开始消失。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study, we used state-of-the-art reversible inactivation techniques to probe in greater detail the interactions between different components of the medial temporal lobe in memory functions in monkeys. These methods permit individual structures within the medial temporal lobe to be selectively turned "off" and "on" for a short period of time while some specific memory functions are tested. These reversible inactivation procedures create "virtual lesions" as the underlying neural circuitry is not structurally disrupted and a particular site is only temporarily deactivated. During the reporting period, we behaviorally trained one adult male monkey to measure recognition memory abilities. In half of the testing sessions the target structure was inactivated with muscimol (GABA agonist), and with the other half, vehicle was injected. Our preliminary data indicate that muscimol achieved maximal behavioral effect between 25 minutes to one hour, after which the effects begin to wear off.