CAPILLARY ELECTROPHORESIS - MASS SPECTROMETRY ON PROTEOMIC ANALYSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在后基因组时代，蛋白质组学已经成为鉴定和量化细胞复杂蛋白质机制的主导领域。蛋白质的表达水平、翻译后修饰和特定的相互作用控制着发育、分化和信号转导等过程的生物学。对参与这些过程的蛋白质的研究往往有助于更好地理解生物学和人类疾病。强大的分离技术和灵敏的检测方法使研究人员能够解开这些复杂的过程网络。CE与MS或LIF相结合是实现这一目标的两种技术。这篇综述将包括细胞和组织水平上基于CE的蛋白质组学方法及其在生物学和临床研究中的应用，使能技术的相关新进展，如在模型系统上演示的微流控CE-MS，并对CE在蛋白质组学中的未来进行评论

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the postgenomic era, proteomics has become a dominant field for identifying and quantifying the complex protein machinery of the cell. The expression levels, posttranslational modifications, and specific interactions of proteins control the biology of such processes as development, differentiation, and signal transduction. Studies of the proteins involved in these processes often lead to a better understanding of biology and of human disease. Powerful separation techniques and sensitive detection methods enable researchers to untangle these complicated networks of processes. CE coupled with either MS or LIF are two of the techniques that make this possible. This review will cover proven CE-based methods for proteomics on the cell and tissue level and their application in biological and clinical studies, relevant new developments in enabling technology such as microfluidic CE-MS demonstrated on model systems, and comment on the future of CE in proteomics