MASS SPECTROMETRY FOR PROTEOMICS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 质谱技术已广泛应用于生物样品的分析，并已发展成为蛋白质组学研究不可或缺的工具。我们对了解蛋白质组的渴望导致了新技术的出现，这些技术推动了质谱分析能力的边界，这反过来又使质谱分析能够解决越来越多的生物学问题。最近发展的一种新型质谱仪（轨道阱）和新的解离方法，如电子转移解离蛋白质组学应用的令人兴奋的新领域。虽然自下而上的蛋白质组学（蛋白水解肽混合物的分析）仍然是蛋白质组学分析的主力，但中间向下和自上而下的策略（分别分析较长的肽和完整的蛋白质）应该可以更完整地表征蛋白质异构体和翻译后修饰。最后，稳定同位素标记策略已经将质谱从仅仅描述性的转变为测量蛋白质表达、相互作用和修饰的动态变化的工具

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mass spectrometry has been widely used to analyze biological samples and has evolved into an indispensable tool for proteomics research. Our desire to understand the proteome has led to new technologies that push the boundary of mass spectrometry capabilities, which in return has allowed mass spectrometry to address an ever-increasing array of biological questions. The recent development of a novel mass spectrometer (Orbitrap) and new dissociation methods such as electron-transfer dissociation has made possible the exciting new areas of proteomic application. Although bottom-up proteomics (analysis of proteolytic peptide mixtures) remains the workhorse for proteomic analysis, middle-down and top-down strategies (analysis of longer peptides and intact proteins, respectively) should allow more complete characterization of protein isoforms and post-translational modifications. Finally, stable isotope labeling strategies have transformed mass spectrometry from merely descriptive to a tool for measuring dynamic changes in protein expression, interaction, and modification