ADVANCED OPTICAL MICROSCOPY IMAGING TECHNIQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 由于其独特的光学性质，量子点（QD）和金纳米颗粒（NP）有望在医学上实现创新的诊断和治疗方法。我们将量子点和金纳米粒子注入新鲜的动物组织。与未注射的图像相比，已获得胶原、血管和肌肉的双光子和SHG图像的质量和分辨率的显著增强。特别是，我们已经能够看到在没有注射QD的情况下保持不可见的不同水平的组织。 在这个项目中，我们的目标是探索以可控的方式将量子点和金纳米颗粒递送到单细胞和组织内的替代策略。金纳米颗粒由于其表面等离子体共振增强的光散射和吸收，在癌症诊断和治疗方面具有巨大的潜力。然后，这些金纳米颗粒可以增加高达拉曼光谱信号的10^10倍。我们的主要目标是获得在生物事件期间分离或浸入组织中的单个细胞的细胞内化学成分（振动键）的指纹，例如，DNA损伤/修复、应力、光消融、降解、机械应力等。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Due to their unique optical properties Quantum Dots (QDs) and gold nanoparticles (NPs) promise innovative diagnostic and therapeutic methods in medicine. We are injecting QDots and gold NPs into fresh animal tissue. A remarkable enhancement on the quality and resolution of two-photons and SHG images of collagen, vessels, and muscle has been obtained in comparison with the images without being injected. In particular, we have been able to see different levels of tissue which remain invisible without the injection of QDs. In this project, we aim to explore alternative strategies for delivery in a controllable way QDs and gold NPs into a single cell and also inside tissue. Gold NPs have immense potential for cancer diagnostic and therapy on account of their surface plasmon resonance enhanced light scattering and absorption. These Gold NPs can then increase up to 10^10 times the Raman spectroscopy signal. Our main goal is to obtain the finger print of the intracellular chemical components (vibrational bonds) of a single cell isolated or immersed into tissue during biological events as for example, DNA damage/repair, stress, photoablation, photoporation, mechano-stress among others.