CORE B: PROGENITOR CELL ANALYSIS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 对于该核心，一个主要功能是提供高水平的流式细胞术能力，以基于免疫学、遗传学、物理和/或代谢特性分析祖细胞群和后代。这些能力在处理罕见的干细胞和祖细胞群体方面非常重要，而且在细胞和生物医学研究中也有很好的应用。具体而言，该核心配备了Becton Dickinson FACS-Calibur流式细胞仪，可进行四色（免疫）染色以及前向和侧向散射分析。它还与专用工作站相结合，该工作站配备高端软件，用于单细胞水平的群体分析，包括细胞周期参数。还支持Flo-Jo、Win-List和相关程序，以促进完整细胞图的出版质量图形。 随着我们中心（和研究所）的持续成功，我们流式细胞仪系统的高效使用显著增加（以至于我们最近申请了缅因州的研发债券基金来购买第二套BD系统）。 对于核心B的这一方面，为了允许ES细胞以及FACS和MACS组件在强有力的初始支持下并行开发，后一套设施和服务（ES细胞、FACS、MACS）已在第6年（通过NCRR批准）重新构建为独立的核心E。这也使得核心B能够开始开发多孔高通量荧光测定法/发光测定法（新获得的模数系统加进样器），并有效地日常使用多功能Vi-Cell细胞分析仪。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide high level flow cytometric capabilities to analyze progenitor cell populations and progeny based on immunological, genetic, physical, and/or metabolic properties. These capabilities are of importance in working with rare stem and progenitor populations, but also find excellent application in cell and biomedical investigations at-large. Specifically, this Core is equipped with a Becton Dickinson FACS-Calibur flow cytometer that enables 4-color (immuno)staining plus forward and side scatter analyses. It's also coupled to dedicated workstations with high-end software for population analyses at the single-cell level, including cell cycle parameters. Flo-Jo, Win-List and related programs are also supported to facilitate publication-quality graphics of complete cytograms. With the continuing success of our Center (and Institute) highly productive use of our flow cytometry system has increased markedly (to the extent that we've recently applied for Maine state R & D bond funds to purchase a second BD system). For this aspect of Core B, and to allow ES cell as well as FACS and MACS components to develop in parallel with strong initial support, this latter set of facilities and services (ES cell, FACS, MACS) have been re-framed in year-6 (via NCRR approval) as separate-standing Core E. This also has allowed Core B to begin to develop multi-well high-throughput fluorimetry / luminometry (newly acquired modulus system plus injectors) and to make efficient day-to-day use of a versatile Vi-Cell cell analyzer.