LSM 710 Zeiss Confocal Microscope

描述（由申请人提供）：共聚焦显微镜核心设施（CMCF）位于达勒姆研究中心（DRC），该中心于2006年开放。CMCF于1996年在UNMC发起，自那时以来已服务于80多名科学家的研究目标。它被医学院、埃普利癌症及相关疾病研究所和UNMC/埃普利癌症中心的科学家广泛使用。CMCF现在维护两个共聚焦显微镜，一个蔡司LSM 410和一个蔡司LSM 510 Meta。2006年，SIG计划资助购买了LSM 510，并由UNMC负责研究的副校长提供资金升级为LSM 510Ma。LSM 510M在2007年初投入使用，在CMCF转移到DRC之后不久。这座包含136个实验室的10层建筑现在将成为DRC I；相邻的塔楼（DRC II）将于5月开放，其中包含额外的98个实验室，一楼将增加核心设施空间。除少数例外情况外，实验室模块的使用仅限于现有和新招募的研究人员，他们拥有活跃的研究补助金（NIH或同等学历），平均每年20万美元/模块。这意味着CMCF可以现实地预期，需要访问CMCF进行NIH资助研究的NIH资助研究人员的数量会增加。在2006年，我们计划继续使用lms410和5.1 m，以应付数目增加的调查人员的需要。然而，只有少数用户愿意使用这一仪器的有限容量。相比之下，LSM 510M几乎一直在使用。在工作人员中增加了一名技术人员，因此我们现在有两名知识渊博的人员在工作日从早上7:30到晚上7:30（或在特殊情况下晚些时候）为调查人员提供协助。在拨款截止日期和会议之前，通常需要等待4-5天才能访问LSM 510M。因此，必须提高我们的能力。我们对LSM 710请求的目标是继续提供最先进的设施，并扩大我们核心的能力，以适应不断增加的NIH用户池。自2005年以来，CMCF的共聚焦使用（hrs）几乎翻了一番，并且还在继续增长，这使得购买LSM 710成为一个关键需求。要求的LSM 710具有类星体光谱检测，34个光谱检测通道和6条激光线（405、458,488,514、561和633 nm）和孵育设备，将允许用户对多个荧光团进行优化的共定位，并方便地获得有关蛋白质-蛋白质相互作用和活细胞中蛋白质运输的信息。本建议书中详细介绍的项目是从11个主要用户和23个次要用户中挑选出来的。

DESCRIPTION (provided by applicant): The Confocal Microscopy Core Facility (CMCF) is housed in the Durham Research Center (DRC), which opened in 2006. The CMCF was initiated at UNMC in 1996 and has served the research goals of >80 scientists since then. It is used extensively by scientists from the College of Medicine, the Eppley Institute for Research in Cancer and Related Diseases and the UNMC/ Eppley Cancer Center. The CMCF now maintains two confocal microscopes, a Zeiss LSM 410 and a Zeiss LSM 510 Meta. In 2006, the SIG program funded the purchase of an LSM 510 which was upgraded to an LSM 510Ma with funds provided by Vice Chancellor for Research at UNMC. The LSM 510M was put into service in early 2007, shortly after the CMCF moved to the DRC. This 10 story building, containing 136 laboratories will now become DRC I; an adjacent tower (DRC II) containing an additional 98 laboratories with added core facility space on the first floor will open in May. With few exceptions, occupancy of the lab modules is limited to current and newly recruited investigators with active research grants (NIH or equivalent) averaging > $200,000/year/module. This means that the CMCF can realistically expect an increase in the number of NIH funded investigators with need to access the CMCF for their NIH funded research. In 2006, we planned to keep both the LSM 410 and 510M in use to cover the needs of an increased number of investigators. However, only a few of our users are willing to use the limited capacity of this instrument. In contrast, the LSM 510M is in almost constant use. A technician was added to the staff so that we now have two knowledgeable people to provide assistance to investigators on weekdays from 7:30 AM to 7:30 PM (or later in special situations). Before grant dead-lines and meetings, there is often a 4-5 day wait to access the LSM 510M. Thus, it is imperative to increase our capacity. Our objective with this request for an LSM 710 is to continue providing state of the art facilities and to expand the capacity of our core to accommodate an increasing pool of NIH users. Confocal usage (hrs) in the CMCF has almost doubled since 2005 and continues to grow, making the purchase of the LSM 710 a critical need. The requested LSM 710 with Quasar spectral detection, 34 spectral detection channels and 6 laser lines- 405, 458, 488, 514, 561 and 633 nm and incubation equipment will allow the users to perform optimized co- localization of multiple fluorophores and to conveniently obtain information about protein-protein interactions and protein trafficking in live cells. The projects detailed in this proposal are selected from those of 11 major users and 23 minor users.