Intelligent Imaging Spinning Disk Confocal Microscope

描述（由申请人提供）：所要求的旋转圆盘显微镜将允许华盛顿大学的核心研究人员使用实时成像技术推进研究。旋转圆盘显微镜可以对活体标本中的荧光图像进行快速采样。由于旋转磁盘系统上的图像捕获速度增加，可以在高分辨率下对非常快速的过程进行成像，包括囊泡运输，线粒体动力学变化，细胞内离子浓度变化，膜融合，细胞骨架重塑事件，细胞快速运动等。最大限度地减少光暴露对于许多活体应用至关重要，以减少潜在的光毒性。转盘显微镜受标本比传统的共聚焦显微镜低得多的光水平，因此非常适合现场成像。该仪器支持的项目包括对发育细胞命运的理解，这将对我们理解出生缺陷和其他遗传疾病、听力损失的分子基础以及对导致结核病的宿主-病原体相互作用的详细研究产生关键影响，结核病是一种使儿童和成人衰弱和致命的感染。

DESCRIPTION (provided by applicant): The requested spinning disk microscope will allow the core researchers at the University of Washington to advance research using live imaging technologies. The spinning disk microscope allows rapid sampling of fluorescent images in living specimens. Because the speed of image capture is increased on spinning disk systems, very rapid processes can be imaged at high resolution, including vesicular trafficking, changes in mitochondrial dynamics, changes in intracellular ion concentrations, fusion of membranes, cytoskeletal remodeling events, rapid movement of cells, etc. Minimizing light exposure is critical for many live applications to reduce potential phototoxicity. Spinning disk microscopes subject specimens to much lower light levels than conventional confocal microscopes, and are thus well suited for live imaging. Projects supported by the instrument include the understanding of developmental cell fate, which will have critical impact on our understanding of birth defects and other genetic diseases, the molecular basis of hearing loss, and a detailed study of the host-pathogen interactions leading to tuberculosis, a debilitating and lethal infection of children and adults.