Support and Development of EMAN for Electron Microscopy Image Processing

描述（由申请人提供）：本申请寻求支持继续发展的EMAN，一个广泛的图像处理套件的电子显微镜。结构生物学是研究单个分子和生物纳米组件的三维结构，以了解它们的功能并将其与细胞过程联系起来。这些研究既用于为生物化学提供物理背景，也用于研究特定的相互作用，例如药物与靶分子相互作用，病毒如何与细胞相互作用或生物分子如何发挥物理功能。EMAN的主要用途是一种称为单粒子分析的技术，该技术将单个分子/组件的数千到数十万个2-D图像合并，以产生高分辨率的3-D重建。EMAN在全球拥有超过1500名用户，包括一个完整的基于OpenGL的3D图形用户界面（GUI），该界面建立在数百种图像处理算法的广泛库上。这项申请寻求继续支持EMAN的发展，并扩大其功能，使其对更广泛的研究人员有用。我们将支持一些新的TEM方法，如螺旋处理，随机圆锥倾斜和单粒子层析成像。此外，我们将EMAN的高级GUI连接到其他几个专业的TEM图像处理软件工具，允许用户无缝地在各种软件包之间来回移动数据，并使用EMAN的高级GUI分析和合并结果。目前，由于软件包之间的标准不同，使用多个软件包的过程非常困难和耗时。我们将建立必要的数据和惯例翻译，使此类处理变得简单。EMAN也开始被用作其他学科软件项目的一个组件。这个应用程序将使我们能够帮助这些开发人员努力扩大EMAN的有用性，以新的生物社区。最后，我们将通过改进文档和扩展EMAN的跨平台支持，为现有用户提供持续的支持。结合起来，这些活动将大大提高这个社区的生产力，并为NIH资助的新用户群体提供有用的工具。这些方法被用于生物学的不同领域，并扩大了我们对神经变性，心脏病和癌症等疾病和病症的理解。 公共卫生相关性：EMAN是一款用于分析电子显微镜三维图像的软件。它被用于广泛的健康相关领域，例如学习药物如何与靶分子相互作用，病毒如何与细胞相互作用，以及对细胞生物化学的物理理解。它扩大了我们对神经退行性疾病、病毒感染和癌症等广泛疾病过程的了解。

DESCRIPTION (provided by applicant): This application seeks support for continued development of EMAN, an extensive image processing suite for electron microscopists. Structural biology is the study of the 3-D structure of individual molecules and biological nanoassemblies for the purpose of understanding their function and relating that function to cellular processes. Such studies are used both to provide a physical context to biochemistry as well as to study specific interactions, such as drugs interacting with target molecules, how viruses interact with cells or how biomolecules physically function. EMAN's primary use is for a technique known as single particle analysis, which merges thousands to hundreds of thousands of 2-D images of individual molecules/ assemblies to produce a high resolution 3-D reconstruction. EMAN has an installed user-base of over 1500 users worldwide, and includes a complete OpenGL based 3-D Graphical User Interface (GUI) built on an extensive library of hundreds of image processing algorithms. This application seeks continued support for the development of EMAN and for expanding its capabilities to make it useful to a broader range of researchers. We will support a number of new TEM methodologies, such as helical processing, random conical tilt and single particle tomography. In addition, we will connect EMAN's advanced GUI to several other specialized TEM image processing software tools, permitting users to seamlessly move data back and forth between the various packages, and use EMAN's advanced GUI to analyze and merge results. At present the process of using multiple software packages is exceptionally difficult and time consuming due to differing standards between the packages. We will establish the necessary data and convention translations to make such processing straightforward. EMAN is also beginning to be used as a component in software projects in other disciplines. This application will permit us to give assistance to these developers working to expand EMAN's usefulness to new biological communities. Finally, we will provide continuing support to our existing users, by enhancing documentation and extending EMAN's cross-platform support. Combined, these activities will serve to greatly enhance the productivity of this community and provide useful tools to new groups of NIH funded users. These methods are used in diverse areas of biology, and have expanded our understanding of diseases and conditions as diverse as neurodegeneration, heart disease and cancer. PUBLIC HEALTH RELEVANCE: EMAN is software for analysis of electron microscopy images in 3-D. It is used in a wide range of health related areas, such as learning how drugs interact with target molecules, how viruses interact with cells and developing a physical understanding the biochemistry of the cell. It has expanded our knowledge of a wide range of disease processes such as neurodegenerative diseases, viral infection and cancer.