ANALYSIS OF TUBULIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 大多数微管蛋白动力学分析和微管蛋白结合蛋白的研究都使用从牛或猪脑中分离出来的微管蛋白。虽然通过反复聚合和解聚可以将微管蛋白从微管相关蛋白中分离出来，但微管蛋白本身有一系列不同的翻译后修饰。我们希望将我们的微管蛋白制剂与商业上可获得的微管蛋白制剂进行比较，如果可能的话，在我们的微管蛋白制剂中寻找翻译后修饰。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Most analyses of tubulin dynamics and the study of tubulin binding proteins use tubulin isolated from bovine or pig brain. Although it is possible to purify the tubulin away from microtubule associated proteins by repeated rounds of polymerization and depolymerization, the tubulin itself has a heterogeneous collection of posttranslational modifications. We would like to compare our tubulin preps with commercially available tubulin preps and if possible look for posttranslational modifications in our tubulin prep.