SPECKLE FIELD PHASE MICROSCOPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 传统的全息位相显微镜(HPM)使用相干光存在三大缺点：空间分辨率低，深度切片弱，以及由于不想要的衍射而导致的固定图案噪声。我们开发了一种技术，可以克服这些缺点，但保持了相位显微镜的优势-高对比度活细胞成像和3D成像。使用复杂空间图案的散斑光束进行照明，以降低固定图案噪声，提高光学分割能力。通过记录散斑的电场，我们展示了高对比度的3D活细胞成像，而不需要轴向扫描-既不需要物镜也不需要样本台。这项技术在研究生物样品方面具有巨大的潜力，可以提高灵敏度、分辨率和光学切片能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. We developed a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrated high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.