LC/MS Metabolomics Core/THOMAS

对细胞、组织和生物体液的低相对分子质量的研究有助于评估人体健康或暴露或药物治疗对生理系统的生化影响。RTI能够为代谢组学研究中广泛使用的基于LC/MS的分析方法提供成熟而独特的广泛经验和专业知识。使用 Jeremey Nicholson博士、Elaine Holmes博士和Ian Wilson博士担任RCMRC的联合研究员和顾问，拟议中的基于LC/MS的代谢组学技术核心将确保客户能够获得可用于数据获取、分析和解释的最新方法。因此，具体的 拟议的LC/MS核心的目的是利用这些关键能力来满足和超过RCMRC的要求及其科学研究人员的服务期望。通过将这些能力和专门知识与其他核心相结合，RTI的LC/MS核心将提供(1)咨询和教育推广服务；(2)进行标准化LC/MS代谢组的分析能力 方法以及新方法和技术的开发和纳入，以满足正在进行和计划中的代谢组学研究的需要，以及(3)以符合DRCC要求和客户需求的方式提供原始数据和汇总统计数据。我们的LC/MS广谱代谢组学方法旨在使用反相和正相(HILIC)来研究非极性和极性分析物 与飞行时间质谱仪联用的层析方法。靶向代谢组学研究在三重四极杆或Q-Trap平台上得到支持，其层析条件针对目标代谢物组进行了优化，并尽可能使用稳定的同位素稀释。RTI致力于提供持续的人员、设施和仪器基础设施来支持这些努力，RCMRC打算继续将广谱代谢组学数据与代谢物鉴定和峰匹配数据进行整合，以生成一个全面的参考数据库，并将其作为代谢组研究社区的资源。

The study of the the low-molecular weight complement of cells, tissues, and biological fluids can be useful in assessing human health or the biochemical effects of exposures or pharmacological treatments on physiological systems. RTI is able to provide a well-established and uniquely broad range of experience and expertise with LC/MS-based analytical methods that are widely used in metabolomics research. With Dr. Jeremey Nicholson, Dr. Elaine Holmes, and Dr. Ian Wilson serving as co-investigators and consultants to the RCMRC, the proposed LC/MS-based metabolomics technology core will ensure that clients have access to the most recent methods available for data acquaition, analysis, and interpretation. Hence, the specific aims of the proposed LC/MS core are designed to leverage these critical capabilities to meet and exceed the requirements of the RCMRC and the service expectations of its scientific researchers. By integrating these capabilities and expertise with other cores, the LC/MS core at RTI will provide (1) consulting and educational outreach services; (2) the analytical capacity for the conduct of standardized LC/MS-based metabolomic methods and the development and inclusion of new methods and technology to meet the needs of ongoing and planned metabolomics studies, and (3) raw data and summary statistics in a manner consistent with the requirements ofthe DRCC and the needs ofthe client. Our LC/MS broad-spectrum metabolomics approach is designed to look at nonpolar and polar analytes using reverse phase and normal phase (HILIC) chromatographic methods coupled to time-of-flight mass spectrometry. Targeted metabolomic studies are supported on triple quadrupoles or Q-trap platforms with chromatographic conditions optimized for the target group of metabolites, and employ stable-isotope-dilution whenever possible. RTI is committed to providing a sustained infrastructure of staff, facilities, and instrumentation to support these efforts, and the RCMRC intends to continuously integrate the broad-spectrum metabolomics data with metabolite identification and peak matching data to generate a comprehensive reference database, and make it available as a resource to the metabolomics research community.