Radio Frequency Probe Development

射频样品探针的改进是高场生物固体核磁共振技术发展的关键。 与溶液NMR不同，固态NMR技术需要将精细的生物样品暴露于非常高的温度。 高强度射频（RF）磁场。除非这些场的电场分量E是 如果最小化，则样品很可能在实验完成之前被加热至破坏。核心 B，我们将应用我们为双频宽孔NMR开发的“低辐射”技术， 三重共振和更容易获得的标准孔磁铁的重要领域。我们还将调整这些 用于样品/流动应用的探头是该提案的核心。 在这个核心中开发的技术不仅需要执行所描述的科学计划， 但它将广泛影响NMR光谱学家进行蛋白质结构和 固体状态下的动力学研究。任何健康和医学领域，受益于更好的 对蛋白质的理解可能会受到影响。

The improvement of RF sample probes is crucial to the development of high field biological solid state NMR. Unlike solution NMR, solid state NMR techniques require exposing the delicate biological sample to very high intensity radio frequency (RF) magnetic fields. Unless the electric field component E of these fields is minimized, the sample will very likely be heated to destruction before the experiment is completed. In Core B, we will apply the "low-E" technology that we have developed for double frequency widebore NMR to the important areas of triple resonance and more accessible standard bore magnets. We will also adapt these probes for the sample/flow applications that are a centerpiece of the proposal. The technology developed in this Core will not only be needed to carry out the scientific program described in the proposal, but it will broadly impact the ability of NMR spectroscopists to conduct protein structure and dynamics studies in the solid state. Any area of health and medicine that benefits from a better understanding of proteins may be affected.