RESEARCH CORE: CORE RESEARCH FACILITIES & INFRASTRUCTURE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 维护和扩展共享研究设施 Brin/INBRE-1开发和加强了一个全州研究设施的综合系统，其中包括三个研究核心实验室、全州生物信息学设施和一个研究变化库，并将得到维护和加强。将为这些共享资源的使用提供便利，并提供全职专家技术援助。INBRE的教职员工和学生将免收或支付使用费。试剂和其他材料的费用将由研究人员通过小额教师发展种子资助来支付，这样教师、博士后或学生只需提供资金购买消耗品。此外，来自所有西方创意国家的研究人员将被邀请使用这些设施 爱达荷大学 A.微阵列设施 1.Biopick机器人 2.BioroBotics 3000工作站 3.微电网机器人 4.GSM417阵列机 5.GenePix 4000a扫描仪 6.Biorad脉冲凝胶电渗疗法 7.高吞吐量PCS 8.实时定量聚合酶链式反应 B.成像核心： 1.IVIS 200(体外成像系统) 2.共焦BioRad 1020(升级和软件) 3.FACS(荧光激活细胞分类器) C.生物信息学核心： 1.接入网格节点教室 2.计算机房 3.集群计算机 4.机器人备份 5.服务器 爱达荷州立大学 D.MCRF(分子核心研究设施) 1.MCRF ABI DNA测序仪服务合同 2.MCRF QPCR升级 3.显微镜/脉管室 4.MRCF组织处理机 博伊西州立大学 E.蛋白质组学核心 1.Gyromax 737R孵化器摇床 2.Gyromax 737孵化器摇床 3.质谱仪(LCQ DECA XP Plus)。购买下列部件的质谱计设备： I.质谱仪/Protemomex Wo 二、东芝1400 XL Plus系列6 三、Proteomex工作站 4.NanoDrop Technologies ND-1000 5.千变万化的IEF系统 6.Biorad外置激光分子成像仪 7.硅图形成像计算机 8.埃彭多夫真空浓缩器5301 9.ALC冷藏离心机 10.努伊尔·胡德x 2 11.Axiovert组织培养显微镜 12.SPM 1000HV控制电子设备，用于RHK控制系统的AFM 100 AFM接口模块，用于分子成像的AIM-MI控制接口和DI(PicoSPM和多节点) 13.Coulter EPICS XL流式细胞仪 14.Jouan冷藏离心机 15.数字仪器(Veeco计量集团)多模PicoForce系统 16.OLIS DM 45K荧光分光光度系统。其中包括以下组件： 一、停流配件经典型号 二、瓦里安分光光度计 17.Cary 50微孔板阅读机 18.Cary 50光纤微探头10 mm路径 19.Dell OptiPlex GX260计算机 20.ABIT 7300聚合酶链式反应循环仪 F.仪器商店共用设施 1.配备Axio观察者Z1共焦成像系统的LSM 510 Meta 405 2.Biotek微型板清洗机 3.柯达IS4000R图像站 4.徕卡CM1950 OUVVM低温恒温器

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Maintain and Expand Shared Research Facilities An integrated statewide system of Research facilities that includes three Research Core Laboratories, statewide Bioinformatics Facilities, and a Research Vivarium was developed and enhanced by BRIN/INBRE-1 and will be maintained and strengthened. Access to these shared resources will be facilitated and full-time expert technical assistance will be provided. User fees will be waived or paid for INBRE faculty and students. The cost of reagents and other materials will be met by the investigator through small faculty development seed grants so that faculty, postdocs, or students need only provide funds for consumable supplies. Additionally, researchers from all the Western IDeA states will be invited to use these facilities University of Idaho A. Microarray Facility 1. Biopick robot 2. BioRobotics 3000 workstation 3. MicroGrid Robot 4. GSM417 arrayer 5. GenePix 4000a scanner 6. BioRad pulsed-gel electophoresis 7. High throughput PCS 8. Real-time PCR B. Imaging Core: 1. IVIS 200 (In vitro Imaging System) 2. Confocal BioRad 1020 (upgrades and software) 3. FACS (Fluorescence Activated Cell Sorter) C. Bioinformatics Core: 1. Access Grid Node classrooms 2. Computer labs 3. Cluster computers 4. Robotic backup 5. Servers Idaho State University D. MCRF (Molecular Core Research Facility): 1. MCRF ABI DNA sequencer service contract 2. MCRF QPCR upgrade 3. Microscope/vessel chambers 4. MRCF Tissue processor Boise State University E. Proteomics Core 1. Gyromax 737R Incubator Shaker 2. Gyromax 737 Incubator Shaker 3. Mass Spectrometer (LCQ DECA XP Plus). Mass Spectrometer equipment purchased in the following components: i. Mass Spectrometer/Protemomex Wo ii. Toshiba 1400 XL Plus Series 6 iii. Proteomex workstation 4. NanoDrop Technologies ND-1000 5. Protean IEF System 6. BioRad External LASER Molecular Imager 7. Silicon Graphics Imaging Computer 8. Eppendorf Vacufuge Concentrator 5301 9. ALC Refrigerated Centrifuge 10. Nuaire Hood x 2 11. Axiovert Tissue Culture Microscop 12. SPM 1000HV Control Electronics, AFM 100 AFM Interface module for use with RHK Control systems, AIM-MI control interface for Molecular Imaging and DI (PicoSPM & Multinode) 13. Coulter Epics XL Flow Cytometer 14. Jouan Refrigerated Centrifuge 15. Digital Instruments (Veeco Metrology group) Multimode PicoForce system 16. OLIS DM 45k Spectroflurimetery system. The following components were included: i. Stop-flow accessory  Classic Model ii. Varian Spectrophotometer 17. Cary 50 Microplate reader 18. Cary 50 Fiber-optic microprobe 10 mm path 19. Dell OptiPlex GX260 computers 20. ABIT 7300 PCR Themocycler F. Instrumentations Shop Shared Facilities 1. LSM 510 Meta 405 with Axio observer Z1 Confocal Imaging System 2. BioTek Microplate Washer 3. Kodak IS4000R Image Station 4. Leica CM1950 OUVVM cryostat