Analytical Cytochemistry

ACC的目标是提供先进的设备和经验丰富的操作人员来测量和分离细胞及其组分的特性，并提供数据供内部分析和外部审查。该核心资源中的流式细胞仪仪器包括（1）高速细胞分选仪（MoFlo）和（2）分析型细胞仪（Cyan和FacsCalibur）。流式细胞仪为研究人员提供了基于多种荧光标记和光散射特性以高达35，000个细胞/秒的速度分析和分离细胞的工具，具有高产量（基于速度高达90%） 纯度极高（99%）。由于能够根据多个参数同时对四个群体进行分类，调查员的后勤和费用大大减少。用于Cyan分析仪的新型高通量平板采样器为拥有大型化合物库的研究人员提供了快速筛选能力，这些研究人员使用流式细胞术分析进行化合物鉴定;激光扫描细胞仪（LSC）的增加扩大了ACC提供的分析能力，包括定量组织分析。 荧光显微镜该仪器收集列表模式荧光数据和显微图像，并为用户提供将定量荧光数据与大组织切片上的定性显微图像相关联的能力。LSC由经过专门培训的用户运行，并由专门的操作员管理。ACC仪器每周进行质量控制评估和例行预防性维护和校准。核心数据库中生成的数据可通过BRI-net服务器获取，以供进一步分析和准备介绍或出版。基于网络的数据处理软件是由核心和实验室信息管理系统（LIMS）正在开发中，以帮助跟踪实验相关的Meta数据和存档文件检索。在2006财政年度，ACC被来自所有5个项目的44名癌症中心成员和3名非结盟成员使用（64%的同行评审使用率）。 这一核心资源的年度预算为401，799美元（43%为机构费用，40%为用户费用）; 17%（70，100美元）由CCSG提供。

The goal of the Analytical Cytometry Core (ACC) is to provide leading-edge equipment and experienced operators to measure properties of, and isolate, cells and their components, and present the data for internal analysis and external review. Flow cytometry instrumentation in this core resource includes (1) high speed cell sorter (MoFlo) and (2) analytical cytometers (Cyan and FacsCalibur). Flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 35,000 cells/second based on multiple fluorescent labels and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Owing to the capability to sort for four populations at a time on multiple parameters, the logistics and cost for the investigators are substantially reduced. A new high throughput plate sampler for the Cyan analyzer provides rapid screening capacity to investigators with large compound libraries who use flow cytometric analysis in compound identification; the addition of a Laser Scanning Cytometer (LSC) has expanded the analytical capacity offered by the ACC to include quantitative tissuebased fluorescence microscopy. This instrumentation collects both listmode fluorescence data and microscopic images and provides users with the capacity to correlate quantitative fluorescent data with qualitative microscopic images on large tissue sections. The LSC is run by specially trained users and managed by a dedicated operator. ACC instrumentation is subject to weekly quality control assessment and routine preventive maintenance and calibration. Data generated in the Core is available through the BRI-net server for further analysis and preparation for presentation or publication. Network-based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment-related meta data and archived file retrieval. During FY 2006, the ACC was used by 44 Cancer Center members from all 5 programs and 3 non-aligned members (64% peer-reviewed usage). Annual budget for this core is $401,799 (43% institutional, 40% user fees); 17% ($70,100) is requested from the CCSG.