ESTABLISHMENT OF SPECIFIC PATHOGEN FREE RHESUS AND PIGTAIL MACAQUE COLONIES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 寄生性人类病毒和它们的猿类对应物在遗传组成上相似，并且在它们的免疫抑制宿主中诱导相似的疾病谱。实验性慢病毒感染恒河猴被广泛认为是研究艾滋病相关机会性感染最重要的动物模型。该项目的目标是通过开发一个扩大定义的SPF印度恒河猴繁殖群，以支持重点研究艾滋病相关的机会性感染，从而加强非人灵长类动物中艾滋病相关研究的基础设施。这些项目旨在通过每年从该中心的SPF印度恒河猴资源中招募经过广泛筛选的青少年雌性并保留雌性后代，将该中心目前的SPF恒河猴种群扩大到100只成年雌性繁殖者的目标种群。具体目标包括：1）建立和管理不含巨细胞病毒、棒状病毒、淋巴隐病毒、猕猴疱疹病毒1、猿猴病毒40、猿猴T淋巴细胞病毒1、D型猿猴逆转录病毒、猿猴免疫缺陷病毒和猴逆转录病毒（泡沫）感染的扩大的SPF印度起源恒河猴繁殖群; 2）维持群体遗传多样性和选定的I类和II类等位基因的主要组织相容性复合体（MHC）分型;和3）更新4，030平方米的英尺的壳空间，以提供室内群体住房和支持区域，与其他猕猴分开，以保护扩大的SPF群体。该殖民地被维持在最先进的屏障住房中，由大约140只成年和幼年动物组成。每年约有24只SPF猕猴可用于研究项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Opportunistic human viruses and their simian counterparts are similar in their genetic makeup and induce a similar spectrum of diseases in their immunosuppressed hosts. Experimental lentivirus infection in rhesus macaques is widely recognized as the most important animal model for AIDS-related opportunistic infection research. The objective of this project is to enhance infrastructure for AIDS-related research in nonhuman primates by developing an expanded definition SPF Indian rhesus macaque breeding colony to support research focused on AIDS-related opportunistic infections. The projects seeks to expand the Center's current population of expanded SPF rhesus macaques to a target population of 100 adult breeder females by annually recruiting extensively screened juvenile females from the Center's SPF Indian rhesus macaque resource and retaining female offspring. Specific aims include: 1) establishing and managing an expanded SPF Indian-origin rhesus macaque breeding colony free of cytomegalovirus, rhadinovirus, lymphocryptovirus, Cercopithecine herpesvirus 1, simian virus 40, simian T lymphotropic virus 1, D type simian retroviruses, simian immunodeficiency virus and spumaretrovirus (foamy) infections; 2) maintenance of colony genetic diversity and major histocompatibility complex (MHC) typing for selected class I and II alleles; and 3) renovation of 4,030 sq. ft. of shell space to provide indoor group housing and support areas separate from other macaques to protect the expanded SPF colony. The colony is maintained in state-of-the-art barrier housing and is comprised of approximately 140 adult and juvenile animals. Approximately 24 expanded SPF macaques are made available for research projects annually.