MR HISTOLOGY OF THE ADULT RAT, COMPARING IN VIVO AND EX VIVO IMAGES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 磁共振组织学已成为神经学研究中定量体积变化的重要工具。我们特别感兴趣的是暴露在酒精中的动物(Wistar大鼠)大脑特定区域的变化。我们目前正在北卡罗来纳大学的金砖四国进行纵向研究，以跟踪这些变化。我们的部分方案涉及体外终点(MR组织学)，以确定相对准确性和重复性(体内与体外)。评价者对体外测量的体积与体内情况的推论的有效性提出了质疑。我们的期望是，体外研究将更准确地衡量体积。组织学先生在金砖四国没有像在CIVM那样得到充分发展。我们将在翻译灌注和扫描方案方面寻求CIVM的帮助。同时我们也想了解一下相关的扫描方法。 动物：灌流固定的鼠头将在北卡罗来纳大学从已经在金砖四国进行了体内和体外扫描的动物中制备。CIVM将提供与北卡罗来纳大学进行的研究相匹配的高分辨率磁共振组织学检查。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Magnetic resonance histology has become an important tool for quantitative volume changes in neurologic studies. We are particularly interested in changes in specific regions of the brain of animals (Wistar rats) exposed to alcohol. We are currently performing longitudinal studies at the BRIC at UNC to follow such changes. Part of our protocol involves end point ex vivo (MR Histology) to determine the relative accuracy and reproducibility (in vivo vs. ex vivo). Reviewers have raised questions over the validity of inferring volumes measured in ex vivo to the in vivo case. Our expectation is that the ex vivo studies will be far more accurate measures of volumes. MR Histology has not been as fully developed at the BRIC as it has at the CIVM. We would seek the CIVM's help in translating the perfusion and scanning protocols. At the same time we would like to understand the relevant scanning methods. Animals supplied by: Perfusion-fixed rat heads will be prepared at UNC from animals that have been scanned (both in vivo and ex vivo) at the BRIC. CIVM will provide high-resolution MR Histology to match the studies performed at UNC.