DEVELOPMENT OF HIGH-PERFORMANCE DIGITAL IMAGING SYSTEMS FOR MESOSCALE IVEM APPS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 为中尺度IVEM应用开发高性能数字成像系统。 这项工作的重点是研究和开发，以改进作为胶片替代品的数字成像技术。这是一项用于高通量、广域中尺度数据采集和计算机集成显微镜的技术。具体地说，我们继续改进用于显微镜生产的8k x 8k超广域镜头耦合CCD相机系统所使用的硬件和软件。在开发直接探测设备(DDD)方面做了大量工作之后，我们继续探索和开发用于瞬变电磁的新电路和抗辐射设备。同样，我们继续进行工程和软件开发活动，以整合新出现的DDD原型，以便在我们的资源IVEM上进行常规数据采集。作为这些系统持续开发和改进的驱动力，我们培育了利用这些设备的性能特征的中尺度显微镜应用程序，并将它们应用于合作研究。这些目标分为以下具体目标：第一，为了适应超广域高通量图像采集的需求，我们继续完善NCMIR 8k x 8k镜头耦合CCD相机系统的控制软件和自动化，该系统在我们的JEM4000#2上调试，以及4k x 4k镜头耦合CCD相机系统在我们的JEM4000#1上调试。第二，为了更准确地记录有关生物标本的信息，我们继续改进技术，使用NCMIR发明的经辐射加固的CMOS相机直接记录图像，并将这些DDD放在各种关键资源平台上。第三，我们利用在现场DDD和透镜耦合系统中获得的开发和集成知识，对Titan FEI 80-300 300keV STEM/TEM进行了主要探测器升级。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DEVELOPMENT OF HIGH-PERFORMANCE DIGITAL IMAGING SYSTEMS FOR MESOSCALE IVEM APPLICATIONS. This effort focuses on research, and development to improve technologies for digital imaging as a replacement for film. This is an enabling technology for high-throughput, wide-field mesoscale data acquisition and computer-integrated microscopy. Specifically, we continue to refine the hardware and software employed with an 8k x 8k ultra-wide-field lens-coupled CCD camera system for production use in microscopy. After significant work in the development of direct detection devices (DDD), we continue to explore and develop new circuitry and radiation-hardened devices for use in TEM. Similarly, we continue engineering and software development activities to integrate emerging DDD prototypes for routine data acquisition on our resource IVEMs. As a driver for the continued development and refinement of these systems, we cultivate mesoscale microscopy applications that exploit the performance characteristics of these devices and apply them to collaborative research. These goals are organized into the following specific aims: first, in order to accommodate the need for ultra-wide-field high-throughput image acquisition, we continued to refine the control software and automation of the NCMIR 8k x 8k lens-coupled CCD camera system commissioned on our JEM4000#2, and the 4k x 4k lens-coupled CCD camera system commissioned on our JEM4000#1. Second, to more accurately record information about biological specimens, we continued to refine technologies to directly record images using radiation-hardened CMOS cameras invented at NCMIR, and to field these DDDs on a variety of key resource platforms. Third, we utilized the development and integrations knowledge obtained in fielding the DDD and the lens-coupled systems to perform a major detector upgrade to the Titan FEI 80-300 300keV STEM/TEM.