NEW PROBES & METHODS FOR CORRELATED LIGHT & EM, LIVE IMAGING, & FUNCT ANALYSIS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 相关光学和电子显微镜、活体成像和功能分析的新探针和新方法的开发和应用 在这个子项目中，我们的努力旨在进一步改进和完善探针和方法，以实现相关的光学和电子显微镜。其中包括非常成功的四半胱氨酸标签/双砷标记技术，被公认为是相关时间推移光和电子显微镜的一种强大和通用的方法，以及mini SOG，这是一种新的基因可编码荧光蛋白，专为蛋白质和大分子复合体的相关光和高灵敏度电子显微镜定位而设计。我们还在继续努力，为相关显微镜开发更多的新探测器。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DEVELOPMENT AND APPLICATIONS OF NEW PROBES AND METHODS FOR CORRELATED LIGHT AND ELECTRON MICROSCOPY, LIVE IMAGING, AND FUNCTIONAL ANALYSIS In this subproject, our efforts are directed at further improving and refining probes and methodologies to enable correlated light and electron microscopy. These include the highly successful tetracysteine tag/biarsenical labeling technology acknowledged to be a powerful and versatile method for correlated time-lapse light and electron microscopy, and miniSOG, a new genetically encodable fluorescent protein designed specifically for correlated light and high- sensitivity electron microscopic localization of proteins and macromolecular complexes. We are also continuing our efforts to develop additional new probes for correlated microscopies.