HIGH THROUGHPUT STRUCTURE DETERMINATION AT CESG

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 真核结构基因组学中心（CESG）正在开发快速NMR筛选方法，用于确定蛋白质稳定性，溶解性和构象状态以及从注释的拟南芥基因组中选择的结构基因组学靶点的配体结合特性。特别强调的是放在蛋白质的序列表明，他们可能含有一个新的折叠，蛋白质与新的功能，或蛋白质可能有一个已知的折叠，但与以前没有相关的折叠功能。我们将利用现有的方法，并开发新的NMR方法的高通量蛋白质结构测定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Center for Eukaryotic Structural Genomics (CESG) is developing rapid NMR screening methods for determining protein stability, solubility, and conformational states as well as ligand binding properties of structural genomics targets selected from the annotated Arabidopsis thaliana genome. Particular emphasis is placed on proteins whose sequences suggest that they may contain a novel fold, proteins associated with novel functions, or proteins likely to have a known fold but with a function not previously associated with that fold. We will use the existing methods and develop novel NMR methods for the high throughput protein structure determination.