ISOTOPE ASSISTED METABOLOMICS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 同位素辅助的差异代谢组学：全球代谢谱高通量分析的新策略。 在过去的10年中，大量的努力致力于开发用于分析生物样品中代谢物的高通量方法。 尽管我们可以用质谱法（MS）检测浓度低至10-18摩尔的分子，并可以使用核磁共振（NMR）区分密切相关的物种，但样本的复杂性严重限制了这些技术在代谢组学研究中的性能。 我们开发了一种新的基于重同位素的策略，最大限度地减少了与传统方法相关的问题，并扩展了全球代谢谱的范围和再现性。 我们目前正在利用这种方法研究基因敲除拟南芥的抗旱机制，并阐明人类红细胞代谢通量的调控。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Isotope-Assisted Differential Metabolomics: a New Strategy for High Throughput Analysis of Global Metabolic Profiles. Over the past 10 years, a considerable effort has been devoted to developing high throughput methods for profiling metabolites in biological samples. Although we can detect molecules at concentrations as low as 10-18 molar with mass spectrometry (MS) and can differentiate between closely related species using nuclear magnetic resonance (NMR), sample complexity has severely limited the performance of these techniques in metabolomics studies. We have developed a new heavy-isotope based strategy that minimizes problems associated with traditional methods and extends the scope and reproducibility of global metabolic profiling. We are currently using this approach to investigate the mechanism of drought resistant in genetic knockouts of Arabidopsis thaliana and to elucidate the regulation of metabolic flux in human erythrocytes.