METABOLIC RESPONSES IN E COLI TO OSMOTIC STRESS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 当在高渗透压培养基中培养时，细胞积累有机渗透压调节剂。最近的证据表明野生型E.大肠杆菌（如致尿路感染菌株CFT 073）具有非调节性转运蛋白，并积累渗透压物质，这在大肠杆菌的研究中还没有得到证实。大肠杆菌K-12。这些观察正在使用NMR和质谱法进行，以确定以前未知的渗透剂，这些渗透剂在缺乏已知的细胞调节系统的细菌中积累到高水平。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cells accumulate organic osmolytes when cultured in high osmolality media. Recent evidence indicates that wild type E. coli (eg uropathgenic strain CFT073) possess osmoregulatory transporters and accumulate osmolytes that have not become evident from studies of E. coli K-12. These observations are being pursued using NMR and Mass Spectrometries in an effort to identify previously unknown osmolytes that accumulate to high levels in osmotically stressed bacteria that lack known osmoregulatory systems.